PATHOGENIC BACTERIA

This research work evaluated the geospatial data need for the design of a good road profile using Global navigation satellite system as a tool that will serve the purpose of safety, capacity and aesthetics considerations Before getting the geospatial for the geometric design of the study area, thorough survey of the site was carried out using the dumping level to obtain the coordinate of the point along the study area, The GNSS was used to obtain the geospatial data of the study area, The design consideration includes: the composition of the traffic, the speed and the management of accessible road. Based on the use, the offset from both sides of the road is determined from the centerline point where the road will be placed. A road offset of 2.85 meters results from the carriageway width of 6.5 meters for the Police station road. A 1.75m-wide shoulder was made available to use as a parking lane, walkway, and covered drain. Ample care was made in the plot to prevent any road segments from crossing in the university of Aerial photography or remote sensing data obtained from space satellites that depict a graphical perspective of the land region are other significant design aids employed for this project. Geospatial data are designed to uniquely specify the positions of features on the earth's surface Geospatial data must be acquired in order to plan, construction, and manage the mad infrastructure, which is vital to humans use. These data come from a variety of sources, BNH including aerial photos, topographic maps, satellite images, and hand-drawn maps. For the collection, processing, and display of geographic data, optimal techniques are applied, which result in significant time and financial savings.

All living things need water and rivers constitute one of the most important sources of water for domestic, industrial and agricultural activities. This study was carried out to evaluate the physicochemical properties and prevalence of pathogenic bacteria in Ogbese River, Ovia North East Local Government Area, Edo State. Water samples (twenty-four) were collected from 12 stations at Ogbese Town, and another 12 stations at Olumoye towns along Ogbese river course during the months of June to December, 2018 and February 2019. Bacteriological analysis involving membrane filtration and multiple tube fermentation techniques were employed to isolate bacterial pathogens. Extraction of genomic DNA, amplification, sequencing and blasting were used to identify bacterial isolates. Antibiogram and curing of bacterial isolates were performed using Kirby-Bauer disk diffusion method. Hemolysin production, serum resistance, Sereny test, Ileal loop assay and suckling mouse tests were carried out on the bacterial isolates. The physicochemical parameters and heavy metals analyses were evaluated using standard methods for water quality. Data were analysed using analysis of variance and unpaired Student’s t test.