F. I OKOLAFOR

This study investigated the isolation and characterization of Lactobacillus species
isolated from Mason’s Natural Probiotics and the production of bacteriocin with
antimicrobial activity against Staphylococcus aureus. The isolate was identified as a Gram-positive, catalase- and oxidase-negative rod that fermented galactose, lactose, and D-fructose without gas formation. Bacteriocin activity was assessed using the agar well diffusion method, while enzyme (trypsin) and hydrogen-peroxide neutralization (sodium pyruvate) tests confirmed the proteinaceous nature of the compound. The absence of blue-black coloration in the potassium iodide–starch assay indicated that no hydrogen peroxide was produced. HPLC analysis revealed the presence of bioactive phenolic and flavonoid compounds, including catechin, naringenin, and caffeic acid, which may contribute synergistically to antimicrobial activity. The bacteriocin demonstrated notable inhibition of S. aureus comparable to some standard antibiotics. These findings indicate that bacteriocin from Lactobacillus sp. possesses strong antibacterial potential and could serve as a safe, natural bio-preservative and alternative to synthetic antimicrobials

This study explored the isolation and identification of Lactobacillus species from Piping rock women Probiotics and the production of bacteriocin with germ-fighting power against Escherichia coli. The bacteria were found to be Gram-positive, rod-shaped, and negative for catalase and oxidase tests. They fermented galactose, lactose, and D-fructose without forming gas. The bacteriocin’s effect was tested using the agar well diffusion method, and enzyme (trypsin) and hydrogen peroxide neutralization (sodium pyruvate) tests confirmed that it was protein-based. The lack of blue-black color in the potassium iodide-starch test showed that no hydrogen peroxide was made. HPLC results showed the presence of active compounds such as catechin, naringenin, and caffeic acid, which may help boost its germ-killing action.

This study investigated the isolation and characterization of Lactobacillus species isolated from Mason’s Natural Probiotics and the production of bacteriocin with antimicrobial activity against Staphylococcus aureus. The isolate was identified as a Gram-positive, catalase- and oxidase-negative rod that fermented galactose, lactose, and D-fructose without gas formation. Bacteriocin activity was assessed using the agar well diffusion method, while enzyme (trypsin) and hydrogen-peroxide neutralization (sodium pyruvate) tests confirmed the proteinaceous nature of the
compound. The absence of blue-black coloration in the potassium iodide–starch assay indicated that no hydrogen peroxide was produced. HPLC analysis revealed the
presence of bioactive phenolic and flavonoid compounds, including catechin, naringenin, and caffeic acid, which may contribute synergistically to antimicrobial activity. The bacteriocin demonstrated notable inhibition of S. aureus comparable to some standard antibiotics. These findings indicate that bacteriocin from Lactobacillussp. possesses strong antibacterial potential and could serve as a safe, naturalbio-preservative and alternative to synthetic antimicrobials.