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Abstract
This study investigated the isolation and characterization of Lactobacillus species isolated from Mason’s Natural Probiotics and the production of bacteriocin with antimicrobial activity against Staphylococcus aureus. The isolate was identified as a Gram-positive, catalase- and oxidase-negative rod that fermented galactose, lactose, and D-fructose without gas formation. Bacteriocin activity was assessed using the agar well diffusion method, while enzyme (trypsin) and hydrogen-peroxide neutralization (sodium pyruvate) tests confirmed the proteinaceous nature of the
compound. The absence of blue-black coloration in the potassium iodide–starch assay indicated that no hydrogen peroxide was produced. HPLC analysis revealed the
presence of bioactive phenolic and flavonoid compounds, including catechin, naringenin, and caffeic acid, which may contribute synergistically to antimicrobial activity. The bacteriocin demonstrated notable inhibition of S. aureus comparable to some standard antibiotics. These findings indicate that bacteriocin from Lactobacillussp. possesses strong antibacterial potential and could serve as a safe, naturalbio-preservative and alternative to synthetic antimicrobials.
compound. The absence of blue-black coloration in the potassium iodide–starch assay indicated that no hydrogen peroxide was produced. HPLC analysis revealed the
presence of bioactive phenolic and flavonoid compounds, including catechin, naringenin, and caffeic acid, which may contribute synergistically to antimicrobial activity. The bacteriocin demonstrated notable inhibition of S. aureus comparable to some standard antibiotics. These findings indicate that bacteriocin from Lactobacillussp. possesses strong antibacterial potential and could serve as a safe, naturalbio-preservative and alternative to synthetic antimicrobials.
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