Blessing OTANIYEN

This study explored the isolation and identification of Lactobacillus species from Piping rock women Probiotics and the production of bacteriocin with germ-fighting power against Escherichia coli. The bacteria were found to be Gram-positive, rod-shaped, and negative for catalase and oxidase tests. They fermented galactose, lactose, and D-fructose without forming gas. The bacteriocin’s effect was tested using the agar well diffusion method, and enzyme (trypsin) and hydrogen peroxide neutralization (sodium pyruvate) tests confirmed that it was protein-based. The lack of blue-black color in the potassium iodide-starch test showed that no hydrogen peroxide was made. HPLC results showed the presence of active compounds such as catechin, naringenin, and caffeic acid, which may help boost its germ-killing action.