VITRO ANTIOXIDANT

The in vitro antioxidant activity of methanolic leaf extract of Azadirachta indica (Dogoyaro) was evaluated to determine its potential role in oxidative stress management. Oxidative stress arises from excess reactive oxygen species, contributing to various chronic diseases and increasing the need for safe, plant-derived antioxidants. The extract was analysed using four standard assays: DPPH radical scavenging, ferric reducing antioxidant power (FRAP), nitric oxide scavenging, and total antioxidant capacity (TAC). Results revealed notable antioxidant properties, with the highest DPPH activity observed at 0.20 mg/mL. Nitric oxide inhibition and reducing power also showed moderate effectiveness, particularly at lower concentrations. Although antioxidant activity remained lower than that of ascorbic acid across all assays, the extract demonstrated
meaningful free-radical-neutralizing ability and electron-donating capacity. The TAC value further indicated the presence of bioactive phytochemicals contributing to overall antioxidant strength. Overall, the findings support the potential of Azadirachta indica leaves as a natural antioxidant source capable of contributing to the management of oxidative stress–related conditions.

Chasmanthera dependens is commonly used in Africa traditional system for the management of several pathologies. This research was designed to asses the phytochemical and antioxidant activity of the methanol extract of Chasmanthera dependens roots. The result of the qualitative phytochemical screening revealed the presence of flavonoid, tannins, Terpeniods, reducing sugars, saponins and proanthovyanindins in the extract. The quantitative phytochemical screening further confirmed the concentration of flavonoid(8.61±0.74) tannins(87.05±1.27), proanthoncyanidins (45.1±1.5) and phenols(199.1±1.9). In vitro antioxidant properties of the extract has antioxidant properties as revealed by it's ferric acid antioxidant power (FRAP), and reducing potential. The phytochemicals in the extract was further identified and quantified by HPLC screening. The HPLC fingerprinting revealed a reported activity of Phytochemical with rich medicinal value. The study also shows that Chasmanthera dependens scavenged DPPH (16.59) reducing power increases in absorbance as the concentration of the plant increased. Conclusively this study provide more information on the medicinal use of Chasmanthera dependens and its good antioxidant properties.