PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF METHANOL EXTRACTS OF CHASMANTHERA DEPENDENS ROOT

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Abstract
Chasmanthera dependens is commonly used in Africa traditional system for the management of several pathologies. This research was designed to asses the phytochemical and antioxidant activity of the methanol extract of Chasmanthera dependens roots. The result of the qualitative phytochemical screening revealed the presence of flavonoid, tannins, Terpeniods, reducing sugars, saponins and proanthovyanindins in the extract. The quantitative phytochemical screening further confirmed the concentration of flavonoid(8.61±0.74) tannins(87.05±1.27), proanthoncyanidins (45.1±1.5) and phenols(199.1±1.9). In vitro antioxidant properties of the extract has antioxidant properties as revealed by it's ferric acid antioxidant power (FRAP), and reducing potential. The phytochemicals in the extract was further identified and quantified by HPLC screening. The HPLC fingerprinting revealed a reported activity of Phytochemical with rich medicinal value. The study also shows that Chasmanthera dependens scavenged DPPH (16.59) reducing power increases in absorbance as the concentration of the plant increased. Conclusively this study provide more information on the medicinal use of Chasmanthera dependens and its good antioxidant properties.
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