LEAF EXTRACT

Gastric damage is a common phenomenon in this part of the world, with about 70%–80% of the population having this issue. These have raised major concerns among experts in the medical field. The project “Investigating the Effects of Aqueous Leaf Extract of Camellia sinensis on Arsenic-Induced Gastric Damage in Adult Wistar Rats” aims to investigate the protective effects of aqueous leaf of camellia sinensis in arsenic-induced stomach damage in adult wistar rats. This study is an experimental research design that involves the use of 30 adult wistar rats, which were randomly divided into six groups (A-F). Group A served as the control group, Group B (Arsenic only), Group C (Arsenic+ Omeprazole), Group D(Arsenic+ low dose of extract), Group E(Arsenic+ high dose of extract) and Group F(Arsenic and left to recover). The rats were given water and feed ad libitum only for 14 days for acclimatization. Thereafter, the five treatment groups were given arsenic trioxide (10mg/kg) orally for 14 days to induce gastric damage. Group B rats were sacrificed for pilot study. Group C received Omeprazole(500mg/kg) for 28 days, Group D was administered low dose of the extract(250mg/kg) for 28 days, Group E received a high dose of the extract 500mg/kg) for 28 days also while the last group was left to recover without administering any extract and thereafter sacrificed by cervical dislocation. The results showed that rats treated with arsenic only had statistically significant decrease in body weight compared to the control group while the rats treated with camellia sinensis (250mg/kg) had statistically significant increase in weight compared to the rats in Group B. The rats in Group B had statistically significant decrease in organ weight compared to the control group. Group D had a significant increase in the organ weight compared to Group B. The antioxidant result showed a significant increase in superoxide dismutase, catalase and gluthaione peroxidase level and a significant decrease in malondialadehyde level in Group C, D and E. The histology results showed crater-shaped ulcers in the rats given arsenic toxin only. Normal mucosal lining was seen in the control group as well as in those given arsenic with omeprazole (the standard anti-ulcer drug). The rats given low and high doses of camellia sinensis showed pitting mucosal lining, with those given low doses having better ameliorative effects compared to those given high dose. The last group (reversal group), which were given only arsenic and left to recover, showed severe mucosal erosion. In conclusion, the research findings suggest that camellia sinensis (green tea) has the potential to mitigate arsenic-induced gastric damage in adult wistar rat.

Annona muricata (soursop) is a tropical plant widely used in traditional medicine for various ailments, yet comprehensive safety data on its effects on vital organs remain limited. This research aimed to investigate the histopathological effects of Annona muricata leaf extract on liver, kidney, testis, and ovaries of albino rats. Twenty-four healthy albino rats weighing 180- 200g were procured from the Animal House of the Department of Anatomy, University of Benin, and maintained under standard conditions with unrestricted access to feed and water. The rats were divided into four groups: Group A (control, n=2) received pelleted feed and distilled water; Group B (n=4) was administered 250mg/kg soursop extract; Group C (n=4) received 500mg/kg; and Group D (n=4) was given 1000mg/kg extract orally via gavage for one month. Following treatment, animals were euthanized, blood samples collected for biochemical analysis, and organs harvested for histopathological examination. Results revealed no significant changes in hematological parameters, liver function tests, or reproductive hormone levels across all groups (p > 0.05). However, kidney function analysis showed significant elevation in sodium (143±3.8 mEq/L) and chloride (107.3±0.5 mEq/L) levels in the highest dose group compared to controls (p < 0.05). Histopathological examination revealed normal architecture in the control group organs. Groups B and C exhibited hepatic steatosis with microvacuolar degeneration, while Group D maintained normal liver histology. All kidney, testis, and ovary sections demonstrated preserved normal architecture across treatment groups. The findings suggest that Annona muricata leaf extract exhibits a complex dose-response relationship, with intermediate doses causing hepatic steatosis while higher doses appear protective. The preservation of reproductive organ integrate and absence of significant biochemical toxicity support the traditional use of soursop, though careful dose optimization and electrolyte monitoring are recommended for therapeutic applications.