COMPOSITION

Urinary tract pathogens are increasingly resistant to conventional antibiotics, prompting interest in plant-derived bioactive agents. This study evaluated the phytochemical profile and antibacterial potential of Cinnamomum tamala bark extracts against selected clinical isolates. Dried bark samples were subjected to aqueous and ethanolic extraction, followed by phytochemical screening using GC–MS analysis. Antimicrobial activity was carried out using ditch plate and agar well diffusion methods, while minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined via agar dilution techniques. The ethanolic extract demonstrated concentration-dependent inhibition, with zones of inhibition ranging from 8.25 ± 4.8 mm at 50 µg/mL to 21.75 ± 2.93 mm at 800 µg/mL, showing significant differences across concentrations (p = 0.034). The aqueous extract exhibited no effect at low concentrations but was active at higher concentration, producing inhibition zones up to 6.50 ± 3.77 mm, significantly different across groups (p < 0.001). MIC results indicated stronger activity for the ethanolic extract, particularly against E. coli (12.5 µg/mL), compared to the aqueous extract, which required higher concentrations (100–200 µg/mL) across organisms. Similarly, ethanolic MBC values ranged between 25–100 µg/mL, significantly lower than the consistent 200 µg/mL required for the aqueous extract. Phytochemical screening revealed alkaloids, flavonoids, tannins, terpenoids, and phenols in both extracts, while saponins and glycosides were exclusive to the aqueous extract, and steroids and resins were unique to the ethanolic extract. GC–MS analysis identified major constituents including Squalene (21.13%), 9- Octadecenoic acid (17.62%), and 13-Octadecenal (16.89%) in the ethanolic extract, while the aqueous extract was dominated by 9-Borabicyclo[3.3.1]nonane (28.24%) and Cyclopropane derivatives (17.04%). These findings highlight the potent antibacterial efficacy of C. tamala ethanolic extract, particularly against E. coli, with activity linked to its terpenoid and fatty acid constituents. The results suggest that C. tamala may serve as a promising source of natural antimicrobials.

The study was carried out to determine the yield, physical and chemical composition of Opepe leaves (Nauclea diiderrichi) De Wild. and T. Durand. The leaves used were harvested and blended, before theleaf Protein Concentrate was extracted using 3 different methods (Heat coagulation , acid coagulation and alum precipitation methods) . The result from the analysis showed that acid coagulation , alum precipitation and heat coagulation methods yielded 2.398% , 3.913% and 6.383% respectively. The yield of bagasse was 23.508%. The result of the proximate analysis showed that the dry matter and NFE were higher in alum precipitation method than in acid coagulation and heat coagulation methods . It also showed that CP , CF and ASH were higher in the acid coagulation method than in the alum precipitation and heat coagulation methods, while EE was higher in the heat coagulation method than in the other two methods . The mineral analysis showed that Mg , Na and P were higher in the heat coagulation method than in the acid coagulation and alum precipitation methods . It also showed that K , Fe and Mn were higher in the acid coagulation method than in the alum precipitation and heat coagulation methods . LPC result of Ca , showed that acid and heat coagulation methods yielded the same amount, which were higher than that of alum precipitation method .