food safety

MICROBIOLOGICAL ASSESSMENT OF SELECTED READY-TO-EAT FRUITS SOLD IN AN OPEN MARKET IN BENIN CITY, NIGERIA.

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Abstract
The potential public health risk associated with ready-to-eat (RTE) fruits sold in open markets was investigated through a microbiological assessment of three popular fruits: Apple, Pineapple, and Watermelon. A total of three samples were purchased from a market in Benin City and analyzed using standard microbiological techniques, including serial dilution, plating on selective media, and biochemical analysis. The mean total bacterial count was highest in Pineapple and lowest in Apple, while the mean total fungi count was highest in Apple and lowest in Watermelon. Bacterial isolates included Staphylococcus aureus, Bacillus spp, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Enterobacter. Fungal isolates included Aspergillus niger, Yeasts, Penicillium species, and Mucor. Antibiotic susceptibility testing revealed isolates with resistance to multiple drugs, including Rocephin, Pefloxacin, and Ampliclox (for Gram-positive bacteria), while showing susceptibility to agents like Ciprofloxacin, Gentamicin, Levofloxacin, Ofloxacin, and Amoxicillin. The presence of these pathogenic and opportunistic micro-organisms confirms the contamination of the RTE fruits, which poses a significant public health hazard. This poor microbiological quality is likely due to unhygienic preparation environments, contaminated water, improper handling, and environmental exposure. Regulatory bodies must enhance surveillance, and vendors should strictly adhere to safe food handling practices. Consumers are advised to ensure proper washing of fruits before consumption.
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co-supervisor

DETERMINATION OF POTASSIUM BROMATE IN UNSLICED BREAD IN EGOR LOCAL GOVERNMENT

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Potassium bromate, a chemical once commonly used as a flour improver, has been banned in many countries due to its harmful health effects, including its potential to cause cancer and organ toxicity. Despite these regulations, concerns persist about its continued use in bread production, particularly in small-scale bakeries. This study investigates the presence and concentration of potassium bromate in unsliced bread samples obtained from various bakeries in Egor Local Government Area, using both qualitative and quantitative analytical methods. Bread samples were collected and categorized based on their loaf sizes, with qualitative analysis conducted to detect the presence of potassium bromate. The results revealed that while some samples showed no reaction, others exhibited a blue-black coloration, confirming the presence of potassium bromate. Quantitative analysis further indicated varying concentrations across the tested samples, with some exceeding the acceptable safety threshold. The highest concentration recorded was 0.04 mg/kg, raising significant health concerns. These findings suggest that despite regulatory efforts, potassium bromate is still being used in some bread production processes, potentially putting consumers at risk. The study highlights the need for stricter enforcement of food safety regulations, increased public awareness, and the promotion of safer alternatives in bread-making. Strengthening routine monitoring and implementing more stringent penalties for non-compliance could help eliminate potassium bromate from the food supply chain, ultimately safeguarding public health.
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co-supervisor

MICROBIOLOGICAL ANALYSIS OF MELON (Citrullus lanatus) SEEDS VENDED IN BENIN CITY, EDO STATE, NIGERI

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This study accessed the microbial analysis of melon seeds purchased from different vendors in New Benin market, Ogida market, Oba market and Uselu market in Benin City, Edo state, Nigeria. Samples were collected in sterile plastic containers and taken to the laboratory for microbiological assessment following standard procedures. The microbiological assessment was carried out using cultural techniques. Identification of the microbial isolates was done using biochemical methods, phenotypic virulence properties were evaluated for the isolates and antimicrobial sensitivity was carried out using agar well diffusion method. The results showed that the bacterial counts ranged from 1.96±0.67 to 3.45±1.15x105 CFU/g. The total coliform counts ranged from 4.73±0.67 to 9.66±0.67 x103 CFU/g. The fungal counts ranged from 1.00±0.00 to 6.00±0.58x103 CFU/g. The bacterial isolated from the melon seed samples were Bacillus sp, Escherichia coli, Pseudomonas sp and Staphylococcus aureus. The fungal isolates identified were Aspergillus flavus, Aspergillus niger, Rhizopus sp, Penicillium chrysogenum, Fusarium sp and Trichoderma sp respectively. The antibacterial susceptibility testing showed that all isolates were susceptible to ciprofloxacin, erythromycin and leofloxacin but were also resistant to pefloxacin, gentamycin, ampiclox, zinnacef, amoxicillin and rocephin. It was also evident that all isolates were found to have an MAR index greater than 0.2 which means that the isolates were all pathogens of public health importance. The study therefore suggests regular surveillance and checks to monitor local vended foods on sale to ensure effective food safety
Supervisor(s)
co-supervisor