FACULTY OF PHARMACY

ANTIBACTERIAL AND ANTI-ADHESION STUDIES OF PROBIOTIC STRAINS AGAINST ESCHERICHIA COLI IN THE PRESENCE OF SUPPOSITORY BASES WITH GC-MS PROFILES OF THE COCULTURE

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Probiotics are products containing live microorganisms which when administered in adequate amount confer health benefits to the host. Lactobacillus can confer therapeutic benefits in aerobic vaginitis and ulcerative colitis. The aim of this study was to evaluate the antibacterial, anti- adhesion, and comparative GC-MS profiling of some probiotic strains against Escherichia coli in the presence of some suppository bases. Gram staining reaction and biochemical test were conducted to authenticate all microorganisms. Double-layer agar method was used for the evaluation of the antibacterial effect of Lactobacillus strains. Solidified albumen was prepared and afterward the weight, surface area, and linearity studies were examined across all microorganisms. The de-adhesion of Lactobacillus strains from solidified albumen was evaluated using Tween 20. Co-cultivation assay of individual Lactobacillus strains against Escherichia coli was determined in the absence and presence of Glycerogelatin, Polyethylene glycol and Theobroma. Survival of Lactobacillus strains in the presence of Escherichia coli and bases was quantified. Adhesion assay of individual Lactobacillus strains against Escherichia coli was determined in the absence and presence of the bases. The anti-adhesion of Lactobacillus strains against Escherichia coli in the absence and presence of the bases was determined. A comparative GC-MS analysis profiled compounds produced in Lactobacillus-Escherichia coli-bases interactions. Regression plots and P-values were determined using one way analysis of variance (ANOVA) via SPSS version 27. Gram staining reaction and biochemical test confirmed Lactobacillus reuteri CF48-3A, Lactobacillus gasseri JV-VO3, Lactobacillus rhamnosus LMS2-1 and Escherichia coli ATCC 25922. Double-layer agar method showed a bacteriostatic range of 30.8 to 31.5 mm. Solidified albumen showed: 1.86 g, 817.15 mm with a strong linearity (R2 = 0.83-0.96) for all microorganisms. Tween 20 exhibited a 50% de-adhesion concentration. There was a significant reduction of Escherichia coli in the presence of Lactobacillus strains (p < 0.001). Similarly, a significant reduction of Escherichia coli in the presence of Lactobacillus strains combined with Glycerogelatin base (p < 0.001) and in combination with Polyethylene glycol base (p = 0.001) were observed. No detectable reduction of Escherichia coli in the presence of Lactobacillus strains combined with Theobroma (p = 0.879). Lactobacillus strains survived (0- 40h) in the presence of Escherichia coli combined with Glycerogelatin (p = 0.001); Polyethylene glycol (p = 0.566) and Theobroma (p = 0.614) respectively. The differences were not significant for the anti-adhesion effect of Escherichia coli in the presence of Lactobacillus strains (p = 0.96). A significant anti-adhesion effect of Escherichia coli in the presence of Lactobacillus strains- Glycerogelatin (p = 0.001) and Lactobacillus-Polyethylene glycol (p = 0.001) were demonstrated. xviii Anti-adhesion effect of Escherichia coli in the presence of different strains of Lactobacillus and Theobroma were consistent with control (p = 0.523). Lactobacillus strains showed poor adhesion in glycerogelatin (p = 0.137) while, polyethylene glycol (p = 0.492) and Theobroma (p = 0.302) showed detectable presence of Lactobacillus strains between 25-48 h. Lactobacillus–Escherichia coli-polyethylene glycol interactions produced compounds such as n-Tetracosanol-1, Hexadecanoic acid, methyl ester, 2-Piperidinone, and exacosane. This study showed glycerogelatin and polyethylene glycol as pharmaceutical formulation bases can positively affect the antibacterial and anti-adhesion properties of Lactobacillus probiotic strains. The study also revealed some bioactive lead compounds for further studies
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co-supervisor

PHARMACY STUDENTS' ENGAGEMENT AND EXPERIENCE IN RESEARCH DURING THEIR ACADEMIC CURRICULAR ACTIVITIES

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Department
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Abstract
Background: Research engagement during academic training helps develop essential attributes such as critical thinking, problem-solving, and evidence-based practice. These factors are fundamental for effective pharmaceutical care delivery. Objectives: The aim of this study was to assess the engagement and experience of pharmacy students in the University of Benin, Benin City in research during their academic curricular activities. Methods: The research was conducted among pharmacy students in the accredited faculty of pharmacy in the University of Benin. This study employed a cross-sectional descriptive design to investigate the extent of pharmacy students' engagement and experiences in research during their academic curricular activities. A stratified random sampling technique was employed to ensure that students from each academic level (100–600L) are proportionally represented. Data was collected using an online survey platform comprising institutional email lists, WhatsApp groups, and other social media platforms commonly used by students and was analyzed using Statistical Package for Social Sciences software version 26 Results: In total, 250 participants were enrolled, revealing a nearly even gender distribution (50.8% male, 49.2% female), with majority aged 18-21 years (48.8%). Regarding research engagement, the majority anticipated engaging in data analysis (22.8%), data collection (14%), and presentations (12.8%), with a smaller proportion planning comprehensive involvement across literature review, data collection, analysis, writing, and presentation (9.6%). Most participants viewed research as beneficial for their future careers. Approximately 43.6% rated it as very beneficial and 25.2% extremely beneficial. However, academic workload was expected to interfere with research participation, as 42.4% agreed and 9.2% strongly agreed. The findings revealed varying levels of participation in research activities. Conclusion: The engagement and experience of pharmacy students at the University of Benin in research during their academic curricular activities were assessed in this study. The findings revealed varying levels of participation in research activities, with some students actively involved while others had limited exposure. Additionally, the perceived benefits of research were also highlighted, including enhanced academic performance and professional growth. However, several challenges, such as academic workload and time constraints, were identified as barriers to research engagement.
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co-supervisor

UNIVERSITY STUDENTS’ PERCEPTION OF COMMUNITY PHARMACIES AND THEIR SERVICES IN EKOSODIN COMMUNITY,EDO STATE

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INTRODUCTION: Community pharmacies play a crucial role in providing healthcare services to the general population including students but there is limited research on how this particular demographic perceives and engages community pharmacies. OBJECTIVE: The study aimed to investigate university students’ perception towards community pharmacies and their health services in Ekosodin community, Benin City, Edo state. METHODS: A cross-sectional study was conducted on 400 university students living in the Ekosodin community. A self-administered questionnaire was the primary tool for data collection and consisted of 3 sections: respondent’s demographics, reasons for/frequency of pharmacy visits and assessment of satisfaction levels and actionable feedback. Collected data were analyzed through both descriptive and inferential statistics using statistical software such as the Statistical Package for Social Sciences (SPSS version 21). RESULTS: Out of 400 respondents, 344 (86%) identified medicine purchases as the main reason for visiting the community pharmacy. The role of the pharmacist was seen as satisfactory with a mean score of 4.10 (maximum of 5) while commonly expected health screening services were blood pressure measurements and malaria parasite tests (253, 63%). The majority (273, 68.3%) were willing to discuss medicines prescribed for them with the pharmacist with a lack of privacy often cited as the main barrier to communication (239, 59.8%). CONCLUSION: The study revealed that the majority of university students were satisfied with the provided health services and perceive community pharmacists as drug experts whom they can willingly discuss medication issues with. However, the lack of privacy in the pharmacy hindered effective communication.
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co-supervisor

PHYTOCHEMICAL INVESTIGATION AND ANTIMICROBIAL ACTIVITY STUDY OF Artemisia annua L. LEAVES (ASTERACEAE)

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Artemisia annua (Asteraceae), commonly known as sweet wormwood, has been widely used in traditional medicine for treating fevers, malaria, and various infections. While it’s active component, artemisinin, is well known for its antimalarial properties, recent studies have suggested that other bioactive compounds in A. annua may also exhibit significant antimicrobial activity. However, the antimicrobial potential of A. annua leaf extract, particularly against multidrug-resistant bacteria, remains underexplored. This study was therefore designed to evaluate the antimicrobial potential of A. annua leaves and identify constituents with potential antimicrobial activity. The research involved the collection and authentication of A. annua leaves, extraction and fractionation using organic solvents of varying polarity (n-hexane, dichloromethane, ethyl acetate, and methanol), acute toxicity screening and antimicrobial testing against selected bacterial strains. Gas Chromatography-Mass Spectrometry (GC-MS) and High-Performance Liquid Chromatography (HPLC) were employed to identify bioactive compounds in the most active fractions. The results showed that the ethyl acetate fraction exhibited the highest antimicrobial activity, with significant inhibitory effects on a broad spectrum of bacteria. Key bioactive compounds
identified include scopoletin, deoxyqinghaosu, naringenin, kaempferol, and sapogenin. Acute toxicity studies revealed a high safety margin for A. annua extract. These findings highlight A. annua’s potential as a natural antimicrobial agent, offering a sustainable and cost-effective alternative to synthetic antibiotics, particularly in resource-limited settings.
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co-supervisor

EVALUATION OF THE ATTENUATING PROPERTIES OF VITAMIN C ON SOME ISONIAZID INDUCED NEUROPATHIES IN RATS

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Abstract
Isoniazid is a widely used drug in tuberculosis treatment regimens. Its application in direct observed therapy short course ”(DOTS) along with other medications has been well documented to be efficacious and effective. However, since its introduction over 70 years ago, it has been found to possess adverse effects as the induction of neuropathies. There are estimates that as many as 10 % of patients receiving isoniazid will develop some form of neuropathy. Introduction of new medications to stop these neuropathies still pose a challenge. Pyridoxine (vitamin B6) is currently recommended with isoniazid therapy to avert induction of neuropathy. Although ,the potential of vitamin C as an antioxidant to prevent induced neuropathies has been suggested based on previous studies, the findings from this study were intended to contribute valuable insights into the potential therapeutic role of vitamin C as an adjuvant to mitigate neuropathic complications in isoniazid- based therapies. Using well-established animal models, we assessed the effects of vitamin C supplementation on the development and progression of some neuropathic symptoms induced by isoniazid administration. Male Wistar rats were divided into six groups: control, isoniazid-treated (800 mg/kg), and combination-treated; Isoniazid with vitamin C in low (7.5 mg/kg), medium (15 mg/kg), high (30 mg/kg) daily doses and isoniazid with pyridoxine (50 mg/kg). Behavioural assessments, including sensory and motor function tests, were conducted at the end of a seven day period to monitor the onset and severity of neuropathy. In conclusion, our findings revealed that isoniazid administration led to a significant decline in sensory and motor functions indicative of peripheral nerve damage. Vitamin C supplementation did not demonstrate a remarkable attenuation of these neuropathic manifestations. Rats co- administered with isoniazid and vitamin C did not exhibit any improvement in sensory and motor functions when compared with the control and standard therapy of pyridoxine. These results negate the potential neuroprotective effects of vitamin C against isoniazid-induced peripheral neuropathy
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co-supervisor

ANTIMICROBIAL STUDIES AND GC-MS ANALYSIS OF AQUEOUS EXTRACT OF Azadirachta indica STEM BARK

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This study evaluated the antimicrobial activity and chemical constituents of the aqueous extract of Azadirachta indica stem bark using standard antimicrobial methods and GC-MS analysis. The antimicrobial activity was tested against clinical isolates, including Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Candida albicans, Klebsiella spp., and Aspergillus niger, using the agar-well diffusion method. The extract, a dark brown substance with a yield of 12%, exhibited inhibition zones ranging from 13 to 40 mm. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified nine bioactive compounds, with 9-Octadecenoic acid (Z)-, methyl ester being the most abundant (64.56%). Other identified compounds include Pyrazine, tetramethyl-; 9- Tetradecenal, (Z)-; Di-n-octyl phthalate; Hexadecanoic acid, methyl ester; Ethanone, 1- cyclododecyl-; Methyl stearate; N,N-bis[2-trimethylsiloxyethyl] ethanamine; and Hexadecanoic acid, 1-[(2-aminoethoxy) hydroxy] derivatives. Some of these compounds have documented antimicrobial properties, which align with the observed inhibitory activity against Gram-positive and Gram-negative bacteria, as well as fungi. These findings support the ethnomedicinal use of Azadirachta indica in managing infections caused by the testedclinical isolates
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co-supervisor

ASSESSMENT OF CURRENT PHARMACEUTICAL CARE PRACTICES BY COMMUNITY PHARMACISTS

Author(s)
Year of Publication
Publication Type
Abstract
Background: Pharmaceutical care is increasingly vital in community pharmacies, where
pharmacists play a key role in patient care. Healthcare reforms and legislative initiatives have driven the shift towards patient-centered care in community pharmacy. Evaluating current practices is crucial for enhancing patient outcomes and continuous assessment ensures alignment with evolving patient needs and standards. Objectives: To assess the current pharmaceutical care practices by community pharmacists and evaluate the extent of patient education practices conducted by study participants in their establishments. Methods: In a cross-sectional observational design to evaluate pharmaceutical care practices among community pharmacists in Benin City, a simple random sampling was employed to select 239 participants. Data was collected through a structured questionnaire and analyzed descriptively using Microsoft Excel 2016. Results: As much as 93.7% of community pharmacists in Benin City reported having a counseling section in their pharmacies. 95.0% actively involved patients in medication management. However, only 45.6% had access to electronic health records, and just 20.9% conducted medication reviews regularly. Concerningly, less than half (49.4%) felt very confident about their knowledge of pharmaceutical care principles. Despite these challenges, over 85% educated patients on the importance of adherence to drug therapy, showcasing their commitment to improving patient outcomes. However, only 54% always provided medication counseling, indicating potential gaps in patient education. Furthermore, while 91.2% felt adequately trained to identify and resolve medication-related problems, only 63.6% considered patients' preferences when providing pharmaceutical care.
Supervisor(s)
co-supervisor

VALUATION OF THE ATTENUATING PROPERTIES OF VITAMIN C ON SOME ISONIAZID INDUCED NEUROPATHIES IN RATS

Author(s)
Year of Publication
Publication Type
Abstract
Isoniazid is a widely used drug in tuberculosis treatment regimens. Its application in “direct observed therapy short course ”(DOTS) along with other medications has been well documented to be efficacious and effective. However, since its introduction over 70 years ago, it has been found to possess adverse effects such as the induction of neuropathies. There are estimates that as many as 10 % of patients receiving isoniazid will develop some form of neuropathy. Introduction of new medications to stop these neuropathies still pose a challenge. Pyridoxine (vitamin B6) is currently recommended with isoniazid therapy to avert induction of neuropathy. Although ,the potential of vitamin C as an antioxidant to prevent induced neuropathies has been suggested based on previous studies, the findings from this study were intended to contribute valuable i sights into the potential therapeutic role of vitamin C as an adjuvant to mitigate neuropathic complications in isoniazid- based therapies. Using well-established animal models, we assessed the effects of vitamin C supplementation on the development and progression of some neuropathic symptoms induced by isoniazid administration. Male Wistar rats were divided into six groups: control, isoniazid-treated (800 mg/kg), and combination-treated; Isoniazid with vitamin C in low (7.5 mg/kg), medium (15mg/kg), high (30 mg/kg) daily doses and isoniazid with pyridoxine (50 mg/kg). Behavioural assessments, including sensory and motor function tests, were conducted at the end of a seven day period to monitor the onset and severity of neuropathy. In conclusion, our findings revealed that isoniazid administration led to a significant decline in sensory and motor functions indicative of peripheral nerve damage. Vitamin C supplementation did not demonstrate a remarkable attenuation of these neuropathic manifestations. Rats co- administered with isoniazid and vitamin C did not exhibit any improvement in sensory and motor functions when compared with the control and standard therapy of pyridoxine. These results negate the potential neuroprotective effects of vitamin C against isoniazid-induced
peripheral neuropathy
Supervisor(s)
co-supervisor

Buniyamin Adesina Ayinde

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Prof Baayinde Ayinde
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ORCID Number: 0000-0002-4259-528X

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Scopus ID
12240561300
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Department of Pharmacognosy,
Faculty of Pharmacy,
University of Benin