EFFECT OF AQUEOUS EXTRACT OF LAWSONIA INERMIS LEAVES ON LEAD ACETATE-INDUCED LIVER DAMAGE IN ADULT WISTAR RATS.
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Abstract
Lawsonia inermis commonly known as henna has been used traditionally, especially in ayurvedic medicine, for various conditions including liver ailments, and reported to have hepatoprotective properties. This study aims to study the effect of aqueous extract of L.
inermis leaves in acute ethanol induced hepatic damage in adult Wistar rats. Thirty (30) female rats were equally divided into five (5) groups (I-V). Group I which served as control received distilled water for 28 days. Group II received Lawsonia inermis for 28 days. Group
III received 100 mg/kg of lead acetate only for 28 days. Group IV simultaneously received 100 mg/kg of lead acetate and treated with 70mg/kg of silymarin for 28 days to serve as reference drug. Group V simultaneously received 100mg/kg of lead acetate and treated with
200mg/kg of L. inermis for 28 days. Group VI simultaneously received 100mg/kg of lead acetate and treated with 400mg/kg of L. inermis for 28 days. Group VII received 400mg/kg of L. inermis and followed with 100mg/kg of lead acetate after 30 minutes for 28 days. All
animals were sacrificed on the twenty ninth (29th) day. Body weight changes and liver body weight index were determined. Liver tissues were collected for assessment of enzymes concentration, and also for haematoxylin and eosin staining. Body weight increased in all
groups from initial mean weight of 180.1 g, though significantly (p<0.05) only in Group IV, Group V and Group VI. Liver body weight index was highest in Group I, and was significantly (p<0.05) different from Group III and Group V. Lead administration reduced level of enzyme aspartate Transferase significantly and increased the value of total bilirubin significantly of (P<0.05) although decrease was seen in enzymes like unconjugated bilirubin, alkaline phosphate and alanine Transferase and increased in conjugated bilirubin but these
were not significant to (P<0.05) H&E staining revealed attenuation of the effects of Lead administration by the extract and silymarin, though the extract proved more effective at 400 mg/kg dose and duration of 28 days. Promising result was also observed in the group that
received 200mg/kg dose over a duration of 28 days.
inermis leaves in acute ethanol induced hepatic damage in adult Wistar rats. Thirty (30) female rats were equally divided into five (5) groups (I-V). Group I which served as control received distilled water for 28 days. Group II received Lawsonia inermis for 28 days. Group
III received 100 mg/kg of lead acetate only for 28 days. Group IV simultaneously received 100 mg/kg of lead acetate and treated with 70mg/kg of silymarin for 28 days to serve as reference drug. Group V simultaneously received 100mg/kg of lead acetate and treated with
200mg/kg of L. inermis for 28 days. Group VI simultaneously received 100mg/kg of lead acetate and treated with 400mg/kg of L. inermis for 28 days. Group VII received 400mg/kg of L. inermis and followed with 100mg/kg of lead acetate after 30 minutes for 28 days. All
animals were sacrificed on the twenty ninth (29th) day. Body weight changes and liver body weight index were determined. Liver tissues were collected for assessment of enzymes concentration, and also for haematoxylin and eosin staining. Body weight increased in all
groups from initial mean weight of 180.1 g, though significantly (p<0.05) only in Group IV, Group V and Group VI. Liver body weight index was highest in Group I, and was significantly (p<0.05) different from Group III and Group V. Lead administration reduced level of enzyme aspartate Transferase significantly and increased the value of total bilirubin significantly of (P<0.05) although decrease was seen in enzymes like unconjugated bilirubin, alkaline phosphate and alanine Transferase and increased in conjugated bilirubin but these
were not significant to (P<0.05) H&E staining revealed attenuation of the effects of Lead administration by the extract and silymarin, though the extract proved more effective at 400 mg/kg dose and duration of 28 days. Promising result was also observed in the group that
received 200mg/kg dose over a duration of 28 days.
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