DEPARTMENT OF ENVIRONMENTAL MANAGEMENT AND TOXICOLOGY

Toxicological Assessment of a widely consumed Nigeria Seasoning powder (BennySeasoning Powder) on Drosophila Melanogaster (Survival, Negative Geotaxis, andNeurotoxicity).

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Benny seasoning is a cooking powder that is commonly used in Africa for improving the taste, aroma and color of food. The potential health risk from the use of Benny seasoning powder remains unclear. Oxidative stress has been implicated in disease onset. Hence in this study, oxidative parameters were assessed in fruit flies (Drosophila melanogaster) exposed to Benny seasoning at various concentrations (Control, 0.025 g/mL, 0.05 g/mL and 0.1g/mL)for seven (7) days. A survival study and climbing assay was conducted and the observations obtained from the study showed that with increasing concentration, mortality rate increased and the climbing activity decreased. After homogenization, specific markers of oxidative stress response (Protein, Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase(GPx), and malondialdehyde (MDA)) were assessed. The results showed that there was no significant difference (p<0.05) when control was compared with the treatment groups. However there were alterations (increase and decrease) in all of the markers indicating a response to oxidative stress. Some genes involved in neurotoxicity were observed (SPITZ, WINGLESS, EIGER, FOXO HEDGEHOG, KEAP1) after exposure to Benny seasoning. There were significant differences with increasing concentrations in the expression of thesegenes indicative of neurotoxicity. Further studies may be needed to fully assess oxidativeeffect at the genetic level in order to completely understand the oxidative stress responses of Drosophila melanogaster to Benny seasoning powder
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ASSESSMENT OF HEAVY METAL CONCENTRATION IN INDUSTRIAL EFFLUENT FROM A CERAMIC INDUSTRY IN BENIN CITY AND ITS ASSOCIATED HEALTH RISK

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Industrial effluents are significant contributors to environmental pollution, especially in developing countries where waste treatment is inadequate. Ceramic industries generate wastewater that often contains toxic heavy metals capable of accumulating in the environment and posing serious health risks. This study assessed the concentration of heavy metals in effluents discharged from a ceramic industrylocated in Utesi, Ikpoba-Okha Local Government Area, Edo State, Nigeria, and evaluated the associated potential health risks. Nine effluent samples were collected from three points around the discharge pond over three months. The samples were analyzed for copper (Cu), chromium (Cr), nickel (Ni), lead (Pb), manganese (Mn),and cadmium (Cd) using the Atomic absorption spectrophotometer following standard procedures. Results showed that Mn had the highest concentration (1.07 3.00 mg/L) in July, while chromium was most abundant in June (0.97–1.00 mg/L). Lead and copper ranged between 0.10–0.47 mg/L and 0.10–0.57 mg/L, respectively, while cadmium reached 0.63 mg/L in August. Compared with the WHO and NESREA standards, Cr, Pb, Mn, and Cd exceeded permissible limits. The mean concentration trend was Mn (1.10 mg/L) > Cr (0.58 mg/L) > Pb (0.27 mg/L) > Cu (0.24 mg/L) > Cd (0.22 mg/L) > Ni (0.06 mg/L). Health risk assessment revealed that cadmium posed the highest non-carcinogenic (HQ = 0.43 for children) and carcinogenic risks (CR = 2.62 × 10⁻⁴ for children).
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INDOOR AIR QUALITY OF LIBRARIES AND LABORATORIES IN A TERTIARY INSTITUTION AND RISK FACTORS FOR RESP

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This study evaluated indoor air quality and examined major risk factors for respiratory symptoms among students who used University of Benin libraries and laboratories. The concentrations of particulate matter, carbon dioxide, and formaldehyde were measured during the morning and afternoon sampling periods, and a structured questionnaire was used to collect demographic, environmental, and health data. Statistical analyses included paired-samples t tests and chi- square tests, with significance set at p < 0.01. The mean pollutant concentrations range from 14.5 ± 0.6 to 24.1 ± 3.1 µg/m³ (PM₂.₅), 27.0 ± 1.5 to 43.2 ± 6.7 µg/m³ (PM₁₀), 399.5 ± 0.3 to 404.4 ± 4.7 ppm (CO₂), and 0.003 ± 0.000 to 0.026 ± 0.012 mg/m³ (HCOH), 24.6 ± 0.2 °C to 32.6 ± 3.7 °C (Temperature), and 69.1 ± 0.5% RH to 78.3 ± 0.6% RH (Relative Humidity).The mean pollutant concentrations did not differ significantly between the morning and afternoon samples, indicating temporal stability. In contrast, environmental factors were significantly associated with symptom prevalence. Notably, a highly significant but inverse relationship emerged for cleaning frequency: participants reporting daily cleaning reported the highest symptom prevalence compared with those reporting monthly cleaning. Demographic variables such as age and sex were not significant predictors of symptoms. These findings indicate that respiratory health risks in these indoor settings are driven less by short-term fluctuations in measured pollutants and more by shortcomings in building maintenance.
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co-supervisor

ASSESSMENT OF DRINKING WATER QUALITY AND HUMAN HEALTH RISK FROM HOUSEHOLDS IN UHOLOR COMMUNITY, OFF UPPER EKEHAUN, BENIN CITY, EDO STATE

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Access to safe drinking water remains a critical public health concern in many developing communities. This study assessed the quality of drinking water and associated human health risks among households in Uholor Community, located off Upper Ekehaun, Benin City, Edo State. Water samples were collected from multiple household sources, including boreholes, wells, and stored water, and analyzed for key physicochemical and microbiological parameters using standard methods.
The results revealed variations in water quality across sources, with several samples exceeding recommended limits for parameters such as pH, turbidity, total dissolved solids, and microbial contamination indicators, including total coliforms and Escherichia coli. The presence of these contaminants suggests potential pollution from environmental and anthropogenic activities, including poor sanitation practices and inadequate waste disposal systems. Health risk assessment indicated that residents may be exposed to waterborne diseases such as diarrhea, typhoid fever, and cholera, particularly among vulnerable populations such as children and the elderly.
Overall, the findings highlight significant concerns regarding the safety of drinking water in the study area. The study recommends regular monitoring of water quality, improved sanitation infrastructure, public health education, and the implementation of appropriate water treatment methods at the household level. Ensuring access to clean and safe drinking water is essential to reducing health risks and improving the overall well-being of the Uholor Community.
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co-supervisor

A COMPARATIVE STUDY ON THE BIOREMEDIATION EFFECT OF PHOSPHATE PSEUDOMONAS SPECIE AND HYDROXYAPATITE ON HEAVY METAL CONTENT IN A MECHANIC WORKSHOP SOIL IN OSASOGIE, BENIN CITY, NIGERIA

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Environmental pollution from heavy metals in mechanic workshop soils poses serious threat to soil fertility, microbial health and groundwater quality. This study evaluated the bioremediation potential of phosphate solubilizing bacteria (Pseudomonas sp.) and hydroxyapatite in reducing heavy metal concentrations in contaminated soil. The study was carried out in a mechanic workshop in Osasogie, Ugbowo, Benin city, Edo state, Nigeria with four treatments: soil only (O1), soil + phosphate solubilizing bacteria (O2), soil + hydroxyapatite (O3) and soil + phosphate solubilizing bacteria + hydroxyapatite (O4). Microbial count and heavy metal concentrations (iron, copper and arsenic) were tested after 14 days. According to results hydroxyapatite was most effective in increasing the growth of Pseudomonas sp. and in the remediation of soil contaminated with copper and iron while phosphate solubilizing bacteria was the least effective due to the short period of remediation. In conclusion, Pseudomonas sp. and hydroxyapatite showed great promise in cleaning up heavy metal contaminated soil from a mechanic workshop.
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co-supervisor

EVALUATION OF HYGIENE STATUS AND QUALITY OF DRINKING WATER FROM HOUSEHOLD IN UHOLOR COMMUNITY. BENIN CITY, EDO STATE

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Access to safe drinking water remains one of the most critical public health challenges in Nigeria, where millions lack reliable sources. Rapid urbanization and inadequate waste management have contributed to the documented water quality challenges in Benin City. However, data on specific localities such as the Uholor community is scarce. In this study, an assessment of drinking water quality based on physicochemical and microbiological parameters was made. Observational checklists were also used to assess environmental conditions and hygiene practices at the household levels. Results have shown that sachet water is the main source of drinking water in the study area, at 52.5%, followed by boreholes at 41.6%. Another high-risk finding is that as many as 69.4% of the households do not treat water before drinking. Though a majority of the respondents, 89.1%, reported the use of flush toilets, environmental sanitation among households is poor 70.3%. The hygiene practices also showed a gap between knowledge and action, as while 91.9% of the respondents were "very" or "somewhat" aware of WASH importance, only 17% reported always using soap to wash their hands. Additionally, 24.6% of households "rarely" clean their water storage containers, entailing a risk of re-contamination. 31.7% of those reporting health issues cited poor taste of water, while 35.4% of households reported illness related to water in the last six months, predominantly Typhoid, 74.3%. The study finds that the community of Uholor is highly exposed to health risks from contaminated drinking water due to widespread non-treatment, and inadequate hygiene practices.
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co-supervisor

HOUSEHOLD WATER QUALITY ASSESSMENT AND HYGIENE PRACTICES IN UPPER MISSION COMMUNITY, OREDO LOCAL GOVERNMENT AREA, BENIN CITY

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Public health and illness prevention depend on having access to clean drinking water and practicing good hygiene. To determine their effects on community health, the Upper Mission community in Benin City's water quality and cleanliness practices were examined. One hundred households chosen by multistage sampling were included in the community-based cross-sectional design. To investigate water sources, storage procedures, treatment techniques, and hygiene practices, structured questionnaires were given out. Additionally, physicochemical and microbiological analyses were performed on 30 drinking water samples obtained from river sources, sachet water, and boreholes. 89% of households have access to water within 100 meters of their homes, with sachet water (52%) and borehole water (41%) serving as the main sources of drinking water. There is a significant gap between accessibility and safety practices, as 80% of individuals did not purify the water before drinking it. With pH values ranging from 4.6 to 8.0, total dissolved solids between 24 and 136 milligrams per liter, and electrical conductivity between 44 and 219 microsiemens per centimeter, the physicochemical parameters mostly satisfied WHO requirements. River water had the highest
contamination (mean: 202.4×10⁰ colony-forming units per milliliter), followed by borehole water (79.7×10⁰ colony-forming units per milliliter) and sachet water (43.2×10⁰ colony-forming units per milliliter), according to microbiological analysis, which found
heterotrophic bacterial counts ranging from 26×10⁰ to 235×10⁰ colony-forming units per milliliter). River water (mean: 42.6×10⁰ colony-forming units per milliliter) and some borehole samples (mean: 24.9×10⁰ colony-forming units per milliliter) had total coliforms, but sachet water did not. Only one sample of river water had Escherichia coli. Although the infrastructure is good, there are notable shortcomings in environmental hygiene, handwashing with soap (34%), and water treatment, with 38% of residences located close to waste disposal sites. There is an immediate need for improved waste management systems, borehole maintenance, targeted health education initiatives, and increased water quality monitoring
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REMOTE SENSING ANALYSIS OF COVID-19 LOCKDOWN EFFECTS ON URBAN HEAT ISLAND (UHI), LAND SURFACE TEMPERATURE (LST) AND GREENHOUSE GASES IN ABUJA.

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The rapid urbanization of the 21st century significantly alters local climates, manifesting in phenomena like the Urban Heat Island (UHI) effect and elevated concentrations of greenhouse gases (GHGs). The COVID-19 lockdown offered a rare natural experiment to evaluate the extent to which human activities influence urban thermal environments and atmospheric conditions. This study employed remote sensing and Geographic Information System (GIS) techniques to analyse the effects of the COVID-19 lockdown on Urban Heat Islands (UHIs), Land Surface Temperatures (LST), and Greenhouse Gases (GHGs) in Abuja, Nigeria. Landsat 8 satellite imagery was used to derive UHI and LST data, while Sentinel-5P provided atmospheric measurements for key GHGs including sulphur dioxide (SO2), aerosols, and ozone (O3). The analysis covered three temporal phases which are the pre-lockdown (2017–2018), lockdown (2019–2020), and post-lockdown (2021–2024) and data were processed using Google Earth Engine and ArcGIS environments to classify spatial variations and identify thermal patterns across the study area. The results revealed a significant decline in both UHI and LST intensity during the lockdown period, with mean UHI values dropping from 6.00°C in 2017– 2018 to 4.93°C in 2019–2020, before rising again to 6.72°C post-lockdown. LST followed a similar trend, decreasing from 6.96°C to 5.14°C during lockdown and increasing thereafter. A corresponding reduction was also observed in atmospheric pollutants, with sulphur dioxide, aerosols, and ozone concentrations all declining during the lockdown. Pearson correlation analysis showed a strong positive relationship between UHI and LST (r = 0.786–0.877), confirming their interdependence and direct link to anthropogenic activity. These findings underscore the dominant role of human activities in shaping urban climatic and atmospheric conditions. The temporary cooling and emission reduction during the lockdown illustrate the potential environmental benefits of reduced fossil fuel consumption and improved urban planning. The study highlights the critical importance of integrating green infrastructure, energy-efficient systems, and climate-responsive policies into Abuja’s urban development framework. It further demonstrates the value of remote sensing and GIS as essential tools for continuous environmental monitoring and policy formulation toward achieving Sustainable Development Goals 11 (Sustainable Cities and Communities) and 13 (Climate Action)
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NORMALIZED DIFFERENCE VEGETATION INDEX (NDVI) EVALUATION OF GAS FLARING ON VEGETATION-A 1991-2013 CASE STUDY OF AWOBA FLOWSTATION, SOUTHERN NIGERIA

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Gas flaring which is the burning of associated gas from crude oil, is a common practice in Nigeria. The absence of a strong regulatory framework, which could be brought about by a lack of awareness of the effects on the environment, is one of the primary reasons for its continuous impacts of gas flaring on vegetation. The study assessed vegetation health with the use of GIS at marked distances of analyzed years. There have been numerous studies conducted to demonstrate the negative impacts of gas flaring on the environment, but none have attempted to measure the cumulative long-term impact on vegetation. Furthermore, there hasn’t been much effort in Nigeria to use GIS to evaluate the effects of gas flaring. The Normalized Difference Vegetation Index (NDVI) a GIS based change detection methodology, was used to examine how the vegetation in the study area changed over time. This was accomplished by gathering four distinct time series of satellite imagery of the research area at specified distances of 1km and 2km, making use of Landsat 4-Thematic Mapper (TM)/1991, Landsat 5-Thematic Mapper (TM)/1999, Landsat 7-Enhanced Thematic Mapper Plus (ETM+)/2003 and Landsat 8-Operational Land Image(OLI)/2013. The results show that gas flaring has a significant role in altering the natural vegetation, with the impact of gas flaring being more pronounced in areas near the flowstation. Furthermore, results from the year 1991 showed that the NDVI values between 1km and 2km did not significantly differ from one another. However, the 1999,2003 and 2013 indicated significant difference in the NDVI values between marked distances. This study emphasizes the need for a safe distance of 2km for human settlement from gas flare stacks. This study recommends that Government should implement policies, such as the polluter pay principle and/or give out incentives to companies so as to reduce gas flaring.
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POTENTIAL OF Cocos nucifera L. WATER IN ALLEVIATING CADMIUM INDUCED ANTIFERTILITY IN MALE WISTAR RATS

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Cadmium (Cd) a toxic non-essential transition metal that poses a health risk for both humans and animals. With many reviews recommending the use of plant extracts in abating heavy metal toxicity due to its rich medicinal properties. This study aimed to investigate the protective effects of Cocos nucifera L. water in abating cadmium-induced toxicity in male wistar rats. Twenty (20) sexually matured male wistar rats were randomly distributed into four groups (n=5). Group A received with 2ml of cadmium chloride, Group B received 2ml of cadmium chloride and 4ml of Cocos nucifera L. water, Group C received 2ml of cadmium chloride and 6ml of Cocos nucifera L. water and Group D received Cocos nucifera L. water for 7 days. Thereafter, rats were sacrificed to obtain the blood and the testis were used for testosterone and histopathological analysis. Result showed that the cadmium chloride significantly decreases (p<0.05) body weight and testosterone level in group A however, the coadministration of Cocos nucifera L. water with cadmium chloride significantly increases (p>0.05) testosterone level both in Group B and C. Histopathological analysis showed that cadmium chloride caused mild intestinal edema in both in Group A when compared with Group D but no significant changes occurred when compared with cotreated groups (Group B and C). From this investigation, Cocos nucifera water showed abating potential in cadmium toxicity due to its polyphenol content and antioxidant properties, however, more studies in recommended
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