COLLEGE OF MEDICAL SCIENCES

This study investigates whether the plant Gongronema latifolium can reduce anxiety (anxiolytic effect) and protect the brain from damage caused by exposure to manganese chloride in laboratory rats.Manganese, although essential in small amounts, becomes toxic when taken in excess. It can accumulate in the brain, leading to oxidative stress, neuronal injury, and behavioral changes such as anxiety and restlessness. Excessive exposure to manganese (Mn) has been associated with neurotoxicity, leading to anxiety-like behaviors due to oxidative stress and neuronal damage. Gongronema latifolium is a medicinal plant commonly used in African traditional medicine for its antioxidant, anti-inflammatory, and protective properties. The research evaluates how effective extracts of this plant are in preventing or reducing anxiety behaviors and brain damage caused by manganese.Wistar rats were exposed to manganese chloride and then treated with different doses of Gongronema latifolium. Their behavior was tested using standard models of anxiety such as the Elevated Plus Maze, Open Field Test, tail suspension test and string test. Brain tissues were later examined for structural and biochemical changes.The study found that Gongronema latifolium reduced anxiety-like behavior, improved antioxidant enzyme levels, and preserved brain structure — showing that it has both anxiolytic and neuroprotective effects. Forty-eight (48) adult Wistar rats of both sexes (n = 8 per group) with an initial mean weight of 100 ± 10 g were randomly divided into six groups. Group A served as the normal control and received distilled water; Group B received manganese chloride (10 mg/kg) to induce anxiety; Groups C and D were treated with manganese chloride plus Gongronema latifolium extract (100 mg/kg and 200 mg/kg, respectively); Group E received Gongronema latifolium (100 mg/kg) as a standard anxiolytic; and Group VI received Gongronema latifolium extract alone (200 mg/kg). Treatments were administered orally for the Gongronema latifolium and intraperitoneally for Manganese chloride for 28 comsecutives days. Neurobehavioral parameters were assessed using the Tail suspension test (TST) ,String Test, Elevated Plus Maze (EPM) and Open Field Test (OFT). Brain tissues were harvested for histological and biochemical analysis.Manganese exposure significantly (p < 0.05) increased anxiety-like behaviors, evidenced by reduced open-arm entries and time in the EPM and decreased central zone exploration in the OFT. Pre-treatment with Gongronema latifolium significantly ameliorated these effects in a dose-dependent manner, comparable to diazepam. In conclusion, Gongronema latifolium demonstrated potent anxiolytic and neuroprotective activities against manganese-induced neurotoxicity through antioxidant and neurorestorative mechanisms. These findings suggest its potential as a natural therapeutic agent for managing anxiety disorders associated with heavy metal neurotoxicity.

Caffeine is a commonly ingested psychoactive substance classified under the methylxanthine group and acts primarily as a central nervous system stimulant. It occurs naturally in beverages and foods such as coffee, tea, cocoa-based products, and numerous energy formulations. Although moderate consumption promotes wakefulness and diminishes tiredness, excessive exposure has been implicated in adverse gastrointestinal outcomes, particularly affecting gastric integrity. This investigation evaluated the impact of graded caffeine administration on the gastric structure and functional indices of adult Wistar rats. A total of twenty adult Wistar rats were randomly assigned to four experimental groups. The control group (Group A) received distilled water, whereas Groups B, C, and D were administered caffeine at doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg body weight respectively for a duration of 21 days. Following the treatment period, the animals were humanely sacrificed, and gastric tissues were harvested for histopathological assessment using Hematoxylin and Eosin (H&E) staining techniques. Morphometric parameters including body weight, gastric weight, and gastrosomatic index were recorded and subjected to statistical analysis. Findings demonstrated a statistically significant (p < 0.05) increase in body weight in all groups relative to baseline values, though weight gain was comparatively reduced in the higher-dose cohorts. Elevated stomach weights and gastrosomatic index values were observed in Groups C and D, suggesting inflammatory changes. Microscopic evaluation revealed preserved gastric histoarchitecture in the control and low-dose groups, whereas the highest dose group (200 mg/kg) exhibited superficial mucosal erosion, vascular congestion within the gastric wall, and submucosal vasodilatation, indicating dose-related gastric injury. Overall, the study establishes that caffeine induces dose-dependent morphological alterations in the gastric tissue of adult Wistar rats. Excessive intake was associated with inflammatory and erosive changes, underscoring the potential gastric risks of high caffeine consumption and supporting moderation in dietary intake.

Background / Purpose: The upper limb function of a stroke survivor following stroke recovery is essential for their functional independence and quality of life. Several studies have been done on the effectiveness of CIMT on upper limb function among hemiplegic stroke patients, however published articles on the effectiveness of CIMT on upper-limb function among hemiplegic stroke survivors in Nigeria are scanty or not available.The study aimed to evaluate the effectiveness of Constraint-Induced Movement Therapy (CIMT) on upper limb function among hemiplegic stroke survivors in a tertiary institution in Benin City. Methods: Simple random sampling technique was used based on the inclusion and exclusion criteria to recruit the 52 participants and they were randomly assigned into a control group and an experimental group. Descriptive and Inferential statistics using One-Way ANOVA Tukey’s post-hoc test to pinpoint specific differences. Alpha level was 0.05. Results: The results showed that CIMT demonstrated significant improvements in upper limb function compared to those who received conventional therapy. The CIMT group showed higher gains in all measured parameters after the eight- week intervention. Statistical analysis revealed a significant difference at p < 0.05 between the groups. Conclusion: The experimental group demonstrated significant increase in muscle strength, muscle endurance, joint flexibility and hand function. Participants also reported higher engagement and motivation throughout the intervention period. These results suggest that CIMT enhances motor recovery and functional performance among stroke survivors, promoting a more effective rehabilitation experience.

The six-minute walk test (6MWT) is a widely-used submaximal field walking test used to evaluate functional exercise capacity. It assesses the distance an individual can walk on a flat, hard surface in six minutes and the final distance is recorded in meters. This study was aimed to investigate changes in oxidative stress marker levels in young adult males of different genotypes post functional exercise at the University of Benin. Forty-four (44) healthy young adult males aged 18-29 years were recruited for this study. Oxidative stress arises from an imbalance between elevated reactive oxygen species (ROS) and insufficient antioxidant defenses Oxidative stress marker levels were measured pre and post-test. The analysis was done at the chemical pathology laboratory of the University of Benin Teaching Hospital, Benin City. The statistical analysis was done using Graph Pad Prism statistical package Version 8.1. The standard error of mean (SEM) was used in tables and graphs to display the results. The dependent and independent variable means were compared using the student t-test. P<0.05 was accepted as significant. The results showed that there was no significant change in the oxidative stress marker levels relative to genotype.

Background: Low back pain (LBP) is a leading cause of occupational disability worldwide, particularly among manual laborers such as bricklayers who are frequently exposed to physical stressors like lifting, bending, and awkward postures. Despite its impact on productivity and health, there is limited local data on LBP among bricklayers in Egor Local Government Area (LGA) of Edo State, Nigeria. Methods: A descriptive cross-sectional survey was conducted among 222 active bricklayers in Egor LGA using a structured, validated questionnaire adapted from the Standardized Nordic Questionnaire. Data were analyzed using the Statistical Package for Social Sciences (SPSS) version 26. Descriptive statistics summarized prevalence data, while Chi-square tests and logistic regression were applied to determine associations between LBP and occupational/personal factors at a significance level of p < 0.05. Results: Findings revealed that the 12-month prevalence of LBP among bricklayers was high. Significant occupational factors associated with LBP included prolonged bending, lifting heavy materials, poor posture, and long working hours. Personal factors such as age, smoking, and elevated body mass index (BMI) also contributed significantly. The majority of affected workers reported recurrent pain episodes that interfered with daily work performance and overall well-being. Conclusion: The prevalence of LBP among bricklayers in Egor LGA is substantial and largely attributable to preventable ergonomic and behavioral factors. Strengthening ergonomic education, enforcing rest breaks, and promoting the use of mechanical aids can reduce the burden of LBP and enhance worker productivity.

BACKGROUND: Food security is essential for human survival, encompassing consistent access to sufficient, safe, and nutritious food. It is defined by four key dimensions: availability, access, utilization, and stability, all of which must be met simultaneously. Food insecurity arises when these dimensions are compromised, leading to inadequate food access. Globally and in Nigeria, its prevalence remains high, driven by factors such as poverty, population growth, and environmental challenges. Food insecurity is associated with adverse health outcomes, including malnutrition and chronic diseases, and negatively impacts education, productivity, and economic growth. AIM: The study aimed to assess the prevalence, determinants, knowledge and perception of food insecurity among households in Benin City, Nigeria and the coping strategies adopted. METHODS: This study employed a community-based descriptive cross-sectional design conducted in Benin City, Edo State, Nigeria, among 500 heads of households. A multistage sampling technique was used to select participants. Data were collected using a pretested, interviewer-administered questionnaire adapted from validated tools, including the Food Insecurity Experience Scale and the Coping Strategies Index. Data analysis was performed using IBM SPSS version 27. Descriptive statistics were presented as frequencies and percentages, while associations were tested using Chi-square or Fisher’s exact tests. Logistic regression was used to identify predictors of food insecurity, with significance set at p < 0.05. Results were presented in prose, tables and bar charts. RESULTS: The mean age of respondents was 51.9 ± 11.9 years, with majority within the 45–54 years age group. The majority 403 (80.6%) were males. The prevalence of food insecurity among households was 383 (76.6%). Food insecurity was higher among respondents aged ≥65 years (86.4%; p = 0.010), married respondents (85.7%; p = 0.004), those with primary education (95.3%; p < 0.001), retired respondents (94.3%; p = 0.017), and those with skill level 2 (85.8%; p < 0.001). It was also higher in households with monthly income ≤ ₦180,000 (84.1%; p < 0.001) and monthly food expenditure ≤ ₦100,000 (82.5%; p < 0.001), and among respondents with good perception of food insecurity (81.5%; p = 0.008). Married respondents had higher odds of food insecurity (OR = 2.135; p = 0.008). Higher education (OR = 0.272; p = 0.017), employment (OR = 0.210; p = 0.012), higher skill level (OR = 0.458; p = 0.001), and home ownership (OR = 0.592; p = 0.040) were associated with lower odds, while increasing number of children increased the odds of food insecurity (OR = 1.236; p = 0.047). Overall, 57.2% of respondents had good knowledge of food insecurity, and 52.0% had a good perception of food security. Knowledge of food insecurity was associated with marital status, where a higher proportion of respondents who were not married had good knowledge (66.4%; p = 0.020). Higher proportions of respondents with monthly household income ≤ ₦180,000 (63.3%; p = 0.003) and those who spent ≤ ₦100,000 on food (61.7%; p = 0.010) had good knowledge of food insecurity. Marital status was associated with lower odds of good knowledge (OR = 0.505; p = 0.007). Higher education increased the odds of good knowledge (OR = 2.351; p = 0.005), while higher skill level reduced the odds of having good knowledge (OR = 0.657; p = 0.036). Perception of food insecurity was associated with level of education, where a higher proportion of respondents with tertiary education had good perception (55.7%; p < 0.001). A higher proportion of respondents with access to a nearby food market also had good perception (55.0%; p < 0.001). Higher education increased the odds of good perception (OR = 3.380; p < 0.001), while access to a nearby food market increased the odds of good perception (OR = 2.777; p < 0.001). Food insecurity status (OR = 1.880; p = 0.004) and good knowledge of food insecurity (OR = 0.515; p = 0.013) were also associated with good perception of food insecurity. The most common coping strategies included consumption of less preferred foods (64.4%), reduction in portion sizes (57.2%), and reduction in the number of meals per day (54.2%). CONCLUSION: The study found that about three-quarters of households were food insecure. Marital status, education, employment, skill level, income, food expenditure, number of children, and housing tenure were important determinants. More than half of respondents had good knowledge and about half had good perception, but many households still used coping strategies such as reducing meal frequency and consuming less preferred foods. The findings reflect widespread food insecurity among households in the study area.

This study examines the influence of exposure to online sexual content on the sexual behaviour of youths in Ugbowo, Benin City. With the increasing accessibility of the internet and digital media, young people are frequently exposed to sexually explicit materials, which may shape their attitudes, perceptions, and practices regarding sexuality. The study adopts a descriptive survey design to assess the level of exposure among youths and its relationship with their sexual behaviour. Data were collected through structured questionnaires administered to selected respondents in the study area. Findings are expected to reveal the extent to which online sexual content contributes to early sexual initiation, risky sexual practices, and changes in sexual attitudes among youths. The study further explores moderating factors such as peer influence, parental guidance, and media literacy. The results aim to provide insights for parents, educators, and policymakers on the need for effective sexual education and digital media regulation to promote healthy sexual development among youths.

Radiography is expected to be a profession where practitioners perform diagnostic imaging procedures while adhering to standard safety protocols, ergonomic principles, and best practices designed to safeguard their physical and psychological well-being. This study set out to evaluate the perceived impact of radiography practices on radiographers working in Edo State hospitals. A total of 95 questionnaires were distributed, with 82 valid responses analysed, representing an 86.3% response rate. The findings revealed that conventional procedures such as chest X-rays, extremities, and abdominal radiography dominate daily workflow, while advanced modalities like MRI and mammography are less frequently performed. Most radiographers reported conducting more than 15 procedures per day, reflecting a heavy workload. The analysis of physical health challenges showed that musculoskeletal complaints, particularly lower back pain, fatigue, and joint or limb pain, were the most significant, while skin and eye irritation was less frequently perceived. On the psychological front, stress, burnout, and overwork due to limited staffing were highly rated, though many radiographers still indicated some degree of job satisfaction. Pearson correlation confirmed a significant positive relationship between radiographic practices and both physical and psychological health outcomes, establishing that workload and procedural demands directly influence the well-being of radiographers in Edo State. The study concludes that radiographic practice in Edo State is marked by a reliance on high- volume conventional imaging, which, while essential for service delivery, has notable consequences on radiographers’ health. The physical burden of repetitive procedures and patient handling, combined with the psychological pressures of heavy workload and limited institutional support, significantly affect professional well-being. Keywords: Practice, Radiography, physical health, musculoskelectal.

Background: Electronic Health Record (EHR) systems are increasingly recognized as essential components of modern healthcare delivery, with the potential to improve patient outcomes, enhance data management, reduce medical errors, and strengthen health system efficiency. Despite these benefits, the adoption and effective utilization of EHR systems in many low- and middle-income countries, including Nigeria, remain low and with influence ranging from multiple individual, institutional, and infrastructural factors. Objective: This study aimed to determine healthcare workers’ knowledge of EHR systems in patient care, assess their attitudes toward EHR utilization, determine uptake and the level of EHR utilization, and identify factors influencing their use across selected hospitals in Benin City, Edo State. Methods: A analytical cross-sectional study design was conducted among 478 healthcare workers selected from public and private hospitals using a multistage sampling technique. Data were collected using a structured, pre-tested, self-administered questionnaire comprising sections on socio- demographic characteristics, knowledge, attitude, utilization, and factors influencing EHR use. Knowledge and utilization scores were categorized based on a 70% cut-off, while attitude was assessed using a 5-point Likert scale which was grouped into appropriate and inappropriate responses and scored using a cut-off of 70%. Data were analyzed using SPSS version 27. Descriptive statistics were summarized as frequencies and percentages, while inferential analysis was conducted using chi-square tests and fisher’s exact and multivariate logistic regression to identify predictors of HER in line with study objectives. Statistical significance was set at p < 0.050, and 95% confidence interval. Results: The majority of respondents were aged 20–39 years (86.8%), with a mean age of 31.22 ± 8.32 years, and females constituted 63.4% of the study population. Nurses formed the largest professional group, and most respondents had less than five years of professional experience. Awareness of EHR systems was high (93.3%), and an equal proportion demonstrated good knowledge (93.3%). Respondents showed strong knowledge in areas such as data privacy, reduction of medical errors, and improvement of healthcare delivery. A majority of respondents (78.2%) had a positive attitude toward EHR utilization, with most agreeing that EHR systems improve patient management (98.1%) and enhance the speed of care delivery (93.3%). However, a considerable proportion expressed neutrality regarding preference for EHR over paper-based records, indicating a transitional phase in adoption. EHR systems were available in most facilities (84.7%), and utilization was high across multiple domains, including clinical documentation (94.1%), patient registration (93.3%), prescribing (92.1%), and laboratory result access (90.6%). Utilization patterns varied significantly by profession and facility type at the bivariate level. Multivariate logistic regression analysis identified knowledge level, sex, and facility type as independent predictors of EHR utilization. Respondents with poor knowledge were significantly less likely to utilize EHR systems compared to those with good knowledge (OR = 0.22; 95% CI: 0.06–0.86; p = 0.029). Male respondents had lower odds of utilization compared to females (OR = 0.32; 95% CI: 0.11–0.89; p = 0.029), while those working in private facilities were less likely to utilize EHR systems compared to those in public facilities (OR = 0.15; 95% CI: 0.03–0.74; p = 0.019). Factors such as training (OR = 5.83; 95% CI: 2.46–13.81; p = <0.001), stability of electricity supply (OR = 7.25; 95% CI: 1.88–27.94; p = 0.004), and availability of hospital policy (OR = 1.99; 95% CI: 1.15–3.47; p = 0.014) were significant predictors of utilization at the multivariate level. Conclusion: This study demonstrates that healthcare workers in Benin City possess relatively high levels of knowledge, positive attitudes, and increasing level of utilization of EHR systems, indicating a strong foundation for digital health integration. However, persistent gaps in training, infrastructure, and organizational support limit optimal utilization. Addressing these challenges through targeted capacity building, improved infrastructural investment, and strengthened institutional policies will be critical for maximizing the benefits of EHR systems and improving healthcare delivery outcomes.

Background The menstrual cycle is a fundamental biological process that introduces considerable hormonal variability, affecting both physiological and psychological functioning in women. Although female participation in sports has grown, research focusing on women’s athletic health remains scarce, especially within African settings where cultural norms and structural barriers often marginalize menstrual health considerations. Aim This research investigated the impact of menstrual cycle phases on physical performance, symptom profiles, and self-management strategies among female athletes at the University of Benin, Nigeria, to generate evidence informed recommendations for cycle aware training and athlete support. Methods Fifty three female athletes participated in this repeated measures study. Physical performance indicators strength, endurance, agility, and reaction time—were evaluated across the menstrual, follicular, ovulatory, and luteal phases. Data on body weight fluctuations and self-reported symptoms were analyzed using repeated measures ANOVA and Pearson’s correlation tests, with significance set at 0.05. Results Significant phase dependent variations in physical performance were observed (p < 0.05). Strength, endurance, and agility were lowest during the menstrual phase, improved during the follicular and ovulatory phases, and declined slightly in the luteal phase. Reaction time was also slower during menstruation. Although body weight remained relatively stable, a significant phase related difference emerged, with slightly lower weight during the menstrual and follicular phases and higher values during ovulation and the luteal phase. A negative correlation was found between changes in body weight and endurance between the ovulatory and luteal phases (r = −0.313, p = 0.023). Frequently reported symptoms included cramps and fatigue during menstruation, bloating during ovulation, and irritability during the luteal phase. Approximately 40 percent of athletes did not track their cycles, and coaches were largely unaware of the performance variations linked to menstrual phases. Conclusion Menstrual cycle phases significantly influence physical performance among female athletes. The conventional “one size fits all” training model fails to accommodate their unique physiological changes. Individualized, cycle sensitive training programs that incorporate menstrual tracking and athlete coach communication are essential to enhance performance, reduce injury risk, and promote well being.