BASIC MEDICAL SCIENCES

ABSTRACT Background: Parental perception and anxiety significantly influence pediatric radiographic procedures, affecting diagnostic quality and patient cooperation. However, limited research exists on this topic in Nigerian tertiary healthcare settings. Aim: This study assessed parental perceptions and anxiety levels related to pediatric radiographic procedures at the University of Benin Teaching Hospital (UBTH), Benin City. Methods: A descriptive cross-sectional survey was conducted among 422 parents/guardians of pediatric patients (aged 0-17 years) undergoing radiographic procedures at UBTH. Data were collected using a structured questionnaire comprising socio-demographic characteristics, a 10-item parental perception scale, and the 6-item State-Trait Anxiety Inventory (S- STAI). Data were analyzed using SPSS version 29, employing descriptive statistics, Pearson's correlation, and chi-square tests at p<0.05 significance level. Results: The response rate was 94.8% (n=400). Mean parental age was 36.4±7.2 years, with 62.5% being mothers. Most parents (68.3%) had tertiary education. The mean perception score was 38.2±5.6 (range: 10-50), indicating moderately positive perceptions. Mean anxiety score was 16.8±4.3 (range: 6-24), reflecting moderate anxiety levels. A significant negative correlation existed between parental perception and anxiety (r=- 0.512, p<0.001). Educational level significantly influenced anxiety levels (χ²=28.45, p<0.001), with less educated parents experiencing higher anxiety. Conclusion: Parents at UBTH demonstrated moderate anxiety levels during pediatric radiographic procedures, significantly associated with their perceptions. Targeted educational interventions are recommended to improve parental understanding and reduce anxiety. Keywords: Parental perception, anxiety, pediatric radiography, tertiary healthcare, Nigeria

Background: Chest X-rays (CXRs) remain the most frequently requested baseline imaging modality in clinical medicine due to their cost-effectiveness, widespread availability, and low radiation dose. Despite the advent of advanced cross-sectional imaging like Computed Tomography (CT), the diagnostic yield and initial investigative value of CXRs in routine clinical practice warrant continuous evaluation.

Dental radiography serves as an essential diagnostic tool for detecting pathologies in the maxillofacial region. This study evaluates radiographic findings, diagnostic accuracy, and prevalence of maxillofacial abnormalities in a Nigerian tertiary healthcare facility. A retrospective review of 264 dental radiographic records from the University of Benin Teaching Hospital (January 2024-January 2025) was conducted. Sociodemographic characteristics, radiographic modalities, clinical findings, pulpal/periodontal diagnoses, and diagnostic accuracy parameters were analyzed using SPSS version 28.0. Descriptive statistics and diagnostic accuracy measures (sensitivity, specificity, positive predictive value, negative predictive value, and overall accuracy) were calculated with 95% confidence intervals. The cohort was predominantly young adults (25-35 years: 43.6%; 52.7% female). Pain was the primary indication for radiography (57.2%). Periapical radiographs were most frequently utilized (59.4%), with all imaging employing digital modality. Dental caries (22.7%) and irreversible pulpitis (17.0%) were the most prevalent clinical and pulpal diagnoses. Cysts or tumors were present in 15.7% of cases, including squamous cell carcinoma (2.2%) and ameloblastoma (1.1%). Radiographic image quality was diagnostic in 98.9% of cases. Diagnostic accuracy demonstrated sensitivity of 87.7-100%, specificity of 83.3-100%, and overall accuracy of 86.7-97.3%, with perfect accuracy (100%) for trauma/fracture detection and 97.3% for tumor/lesion detection. Well-defined lesion borders predominated (81.8%), suggesting predominantly chronic pathologies. Dental radiographs demonstrate substantial diagnostic utility for maxillofacial assessment in this tertiary facility, with high technical quality and diagnostic accuracy. The significant prevalence of caries, periapical pathology, and detected malignancies underscores the importance of radiographic screening protocols. Digital implementation and appropriate modality selection enhance diagnostic capability while supporting radiation safety principles. However, inherent two-dimensional limitations necessitate three-dimensional imaging for complex cases.

Antibiotic resistance is one of the global health concern, as commensal bacteria such as Escherichia coli could serves as an important reservoirs of the spread of resistance genes. This study evaluated the relationship between Body Mass Index (BMI) and antibiotic resistance of commensal Escherichia coli isolated from the stool samples of apparently healthy students at the University of Benin (UNIBEN). A total of 70 stool samples were collected and processed using standard microbiological methods. The isolates were identified by biochemical tests, and antibiotic susceptibility testing was performed using the Kirby– Bauer disc diffusion method in line with CLSI guidelines. The Extended Spectrum β- Lactamase (ESBL) production was screened phenotypically. The overall prevalence of Escherichia coli detected across all BMI categories was 85.7%. The ESBL-producing Escherichia coli accounted for 16.7% of isolates, with a higher proportion among underweight participants (26.1%) compared to those of normal weight (11.8%), though this difference was not statistically significant (p = 0.2984). Antibiotic susceptibility testing revealed that Ofloxacin was the most effective antibiotic, while Cefotaxime showed the highest resistance rates. Statistical analysis demonstrated no significant association between BMI and antibiotic resistance patterns (p > 0.05), although a borderline association was observed with Cefotaxime (p = 0.0523). The findings indicate that while BMI may not be a major determinant of Escherichia coli resistance carriage, healthy students can harbor resistant and ESBL-producing strains that pose a potential public health risk. Regular surveillance and strict antibiotic stewardship are therefore recommended.

Icacina trichantha is widely recognized for its medicinal properties, including antioxidative and hematoprotective effects. Over the past decade, interest has grown in exploring the impact of medicinal plants on gene regulation and hematological parameters.The aim of this study is to investigate the expression of the Signal transducer and activator of transcription-5 (STAT5) gene in aluminium chloride-induced anaemia bearing Wistar rats treated with Icacina trichantha aqueous leaf extract. A total of sixty (60) adult male albino Wistar rats were divided into six (6) groups; A, B, C, D, E and F representing control, aluminum chloride group, ferrous sulfate group, aluminum chloride + 100mg/kg Icacina trichantha, aluminum chloride + 200mg/kg Icacina trichantha and aluminum chloride + 400mg/kg Icacina trichantha respectively. 5 milliliters (5ml) of blood sample were drawn from each rat, and haematological parameters and mRNA of STAT5 was determined using ERMA haematology autoanalyzer and polymerase chain reaction respectively. Data obtained was analyzed using the GraphPad prism software. The comparison of haematological parameters amongst the study groups showed that Mean cell volume (MCV) (µm3) was significantly lower in group F (54.64±0.96) when compared to group C (58.22±0.49) (p<0.05). Mean cell haemoglobin (MCH) (pg) was significantly lower in group F (18.72±0.23) when compared to group C (19.66±0.07) (p<0.05). Platelet distribution width (PDW %) was significantly lower in group E (8.96±0.27) when compared to group D (10.6±0.31) (p<0.05). Groups B, C, D, E, and F showed significantly higher mRNA expression of STAT5 when compared to group A (p<0.05). Groups D, E and F had a higher mRNA expression of STAT5 when compared to group A and B (p<0.05). Group F also had a higher mRNA expression of STAT5 when compared to group D (p<0.05). In conclusion, administration of aluminum chloride and Icacina trichantha aqueous leaf extract led to significant effects on some haematological parameters although the mRNA expression showed dose-dependent alterations in the gene expression of STAT5.

Introduction: The consumption of psychoactive substance remains a significant global health challenge, particularly among young adults in tertiary institutions. Medical students occupy a uniquely precarious position due to the rigorous cognitive and emotional demands of their training. In Nigeria, national drug use prevalence has surged beyond the global average, with synthetic drugs and new psychoactive substances becoming increasingly accessible in southern urban centers like Benin City. Objectives: The aim of the study was to assess the prevalence, pattern and perceived factors associated with substance use among medical students at the University of Benin. Methodology: A descriptive cross-sectional study was conducted among undergraduate medical students (200-level – 600-level) at the University of Benin. A systematic random sampling technique was employed to select the sample size. Data were collected using a structured, self-administered questionnaire adapted from the WHO Student Drug Use Questionnaire (SDUQ) and the General Health Questionnaire (GHQ-12). Analysis was performed using IBM SPSS version 27. Results: A total of 298 respondents participated (100% response rate). Alcohol was the most prevalent substance, with a lifetime usage of 63.4% and a past 30-day prevalence of 5.8%. Tobacco/nicotine had a lifetime prevalence of 14.1%, while current opioid use was reported by 2.7% of respondents. Significant associations were found between alcohol use and academic level (highest in 300-level), monthly allowance, and marital status. Furthermore, alcohol and stimulants were significantly associated with psychological distress (p= 0.024 and p= 0.036, respectively), with 82.0% of current stimulant users exhibiting distress. Conclusion: Substance use is highly prevalent among medical students at the University of Benin, primarily serving as a functional coping mechanism to manage intense academic workloads and systemic rigors rather than recreational purposes. The findings highlight a critical need for institutions to transition from punitive measures towards a wellness-oriented framework, incorporating confidential counselling and curriculum restructuring to mitigate student stress and promote healthier learning environments.

Stimulants are a major constituent of psychoactive substances. They cause several untoward effects; including academic difficulty which can lead to untoward consequences for students. The understanding of the knowledge and pattern of use of stimulant will help in its prevention and control. Thus, this study intended to investigate the knowledge and practice and current prevalence rates of stimulant use, using the undergraduate students in the university of Benin as case study, age and gender of students as well monthly income and how they got to know about stimulants, specific type of stimulant used and reason for using stimulants. The instrument consisted of sociodemographic variables and the stimulant knowledge section, benefits, health effects and effects section of Questionnaire for Student Drug Use Survey. It was administered on 100 undergraduate students of University of Benin selected by random technique. The current prevalence rates of stimulant use were 61%, 54% and 50% respectively for the use caffeine, alcohol and cannabis. The majority (77%) of our respondents were in the age range of 21-25. Only 2% of our respondents were married. Therefore, efforts at curtailing the use of stimulants must start early; incorporating such strategy into primary school curriculum will be worthwhile. There is need to focus more on common stimulants like coffee, Kola nut, caffeinated energy drinks. Students need to study without using stimulants.

This study investigates the factors influencing career pathway choice among final year students of radiography in the University of Benin. A cross-sectional study design will be employed, using a mixed-methods approach to collect data from students. The study aimed to identify the most important factors influencing career pathway choice and explore the relationship between demographic characteristics and career pathway preferences. The findings of this study will contribute to the existing body of knowledge and provide insights into the factors that influence career pathway choice among radiography students, informing educators, professionals, and policy makers. The study's results will have implications for career guidance programs, education, and policy decisions related to radiography and healthcare.

Healthcare policies are central to the regulation of radiographic practice and play a vital role in shaping the quality of patient care. This study investigated the impact of healthcare policies on radiographers at the University of Benin Teaching Hospital (UBTH), focusing on policy awareness, influence on daily practice, patient care outcomes, and the challenges of policy adherence. A descriptive cross-sectional design was used, and data were collected from viii 31 radiographers through a structured questionnaire. The results showed that 61.3% of respondents were aware of institutional healthcare policies, while 74.2% could identify policy documents relevant to their duties. Full compliance with radiation safety requirements (100%) was observed, demonstrating strong adherence in safety-sensitive areas. Policies were found to substantially influence professional conduct, as 93.5% agreed that policies guide daily radiographic practice, while 87.1% indicated that policies ensure standardization and 90.3% affirmed improved accountability. In terms of patient care, 93.5% agreed that policies enhance service quality and 87.1% believed they promote consistency, although only 42.0% felt that policies reduce imaging time. A Chi-square test further confirmed a statistically significant relationship between healthcare policies and daily radiographic practice (χ² = 61.29, df = 1, p = 0.000), leading to the rejection of the null hypothesis. Despite these positive outcomes, radiographers reported major challenges such as inadequate resources (87.1%), insufficient training (61.3%), outdated guidelines (58.1%), and inconsistent implementation across departments (58.0%). The study concludes that healthcare policies positively shape radiographic practice and patient care at UBTH, but their effectiveness is limited by systemic and administrative constraints. Strengthening policy communication, updating guidelines, improving resource availability, and expanding training opportunities will enhance policy adherence and further improve patient outcomes in radiography.

The application of plants as herbal medicine has been used by various populations throughout human evolution, whereas people started to learn in selecting plants for food, to cure and prevent ailments and diseases. The aim of this study is to investigate the effects of aqueous extract of Cinnamon bark on the liver of wistar rat. Twenty (20) Adult Wistar rats of weighing between 150 g-180g were used for this experiment. The rats were procured from the animal holdings of Department of Anatomy, School of Basic Medical Sciences, University of Benin, Benin City. The animals were housed in the Department of Anatomy, University of Benin. Care and management of animals was carried out in accordance with the guidelines for the care and use of laboratory animals. The animals were allowed to acclimatize for a period of two weeks before commencement of experiment. The bark of Cinnamon was purchased from the vegetable market of “Stop to Shop” located opposite the University of Benin. The bark was chopped into little bits and allowed to dry at room temperature. The dried bark was pounded using wooden mortar and pestle and milled into fine powder in an electric blender. Five hundred grams (500g) of the powder were soaked in 2 liters of distilled water for 24 hours. The mixture was filtered with white filter paper and the residue would be separated from the filtrate. The filtrate was concentrated using Freeze dried technique in the National Centre for Energy and Environment at the University of Benin, Benin City. The rats were randomly assigned into a Control group (A) and three (3) treatment groups (B, C, and D) containing five (5) animals each. Rats in Group B were administered with 200 mg/kg aqueous extract of Cinnamon bark; group C was administered with 500 mg/kg aqueous extract of Cinnamon bark; Group D 1000 mg/kg aqueous extract of Cinnamon bark. At the end of the experimental period, the animals were sacrificed using Chloroform anesthesia and the Liver tissues were harvested and fixed in 10% buffer saline for routine histological procedure for hematoxylin and Eosin staining technique. Blood samples were collected from the descending abdominal aorta and preserved, in heparin coated tubes, for biochemical determination of Liver function tests. The data generated carried out using Statistical package for Social Sciences (SPSS) (version 16) manufactured by international Business Machine Corporation (IBM) in Armonk, New York. The significance of the difference in the means of all parameters would be determined using one-way analysis of variance (95% confidence interval). Result obtained showed that the extract had no deleterious effect on the Liver of the rats. However there was activation of inflammatory cells aggravating toward the portal area. Further studies should be carried out to corroborate this finding