AWOMEJU ISRAEL OLUWAYOMI

Antibiotic resistance is one of the global health concern, as commensal bacteria such as Escherichia coli could serves as an important reservoirs of the spread of resistance genes. This study evaluated the relationship between Body Mass Index (BMI) and antibiotic resistance of commensal Escherichia coli isolated from the stool samples of apparently healthy students at the University of Benin (UNIBEN). A total of 70 stool samples were collected and processed using standard microbiological methods. The isolates were identified by biochemical tests, and antibiotic susceptibility testing was performed using the Kirby– Bauer disc diffusion method in line with CLSI guidelines. The Extended Spectrum β- Lactamase (ESBL) production was screened phenotypically. The overall prevalence of Escherichia coli detected across all BMI categories was 85.7%. The ESBL-producing Escherichia coli accounted for 16.7% of isolates, with a higher proportion among underweight participants (26.1%) compared to those of normal weight (11.8%), though this difference was not statistically significant (p = 0.2984). Antibiotic susceptibility testing revealed that Ofloxacin was the most effective antibiotic, while Cefotaxime showed the highest resistance rates. Statistical analysis demonstrated no significant association between BMI and antibiotic resistance patterns (p > 0.05), although a borderline association was observed with Cefotaxime (p = 0.0523). The findings indicate that while BMI may not be a major determinant of Escherichia coli resistance carriage, healthy students can harbor resistant and ESBL-producing strains that pose a potential public health risk. Regular surveillance and strict antibiotic stewardship are therefore recommended.