PLASMID CURING OF MULTI-DRUG RESISTANT BACTERIAL ISOLATES FROM PATIENTS WITH SINUSITIS
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Abstract
The nasal cavity hosts a wide array of organisms. Pathogenic organisms may find access into the sinuses leading to infection. Plasmids, which are small extracellular DNA molecules, play a role by transferring resistance encoded genes among bacteria, facilitating the spread of antibiotic resistance. By investigating the role of plasmid genes in potential treatment failures, the research seeks to broaden our understanding on how plasmids mediate
antibiotic resistance. This knowledge is key for developing effective strategies to combat
sinusitis disease using anti microbialagents.
Methods: The study evaluated forty-three patients from the Ear Nose and Throat Clinic of the University of Benin Teaching Hospital, Benin city. Patient’s data collected were age,gender, alcoholhistory, smokinghistory, medical history. Isolates obtainedwere subjected to antimicrobial susceptibility testing and plasmid curing with acridine orange as the curing agentusingstandardagardiscdiffusionmethod. Results: A total of 58.1% of our study participants were female, and 41.9% were male. Participants aged 16-25 had the highest sinusitis occurrence (27.9%). Nasal discharge was the most encountered symptom across all participants. Staphylococcus aureus dominated in
the nasal cavity of study participants (35.1% aerobic) compared to other organisms isolated. Pseudomonas aeruginosa accounted for the highest abundance (31.3%) under anaerobic condition. Multi-drug resistance was observed in 23.4% and 25% of total aerobic and anaerobic isolates respectively. Resistance to fluoroquinolones was lost post curing in Staphylococcusspp,Klebsiellaspp.
Conclusion: Females were more susceptible to sinusitis with recurrent episodes experienced compared to males. The presence of plasmid-mediated multidrug resistance genes
underscores the need for antibiograms, and rational antibiotics use insinusitis treatment.
antibiotic resistance. This knowledge is key for developing effective strategies to combat
sinusitis disease using anti microbialagents.
Methods: The study evaluated forty-three patients from the Ear Nose and Throat Clinic of the University of Benin Teaching Hospital, Benin city. Patient’s data collected were age,gender, alcoholhistory, smokinghistory, medical history. Isolates obtainedwere subjected to antimicrobial susceptibility testing and plasmid curing with acridine orange as the curing agentusingstandardagardiscdiffusionmethod. Results: A total of 58.1% of our study participants were female, and 41.9% were male. Participants aged 16-25 had the highest sinusitis occurrence (27.9%). Nasal discharge was the most encountered symptom across all participants. Staphylococcus aureus dominated in
the nasal cavity of study participants (35.1% aerobic) compared to other organisms isolated. Pseudomonas aeruginosa accounted for the highest abundance (31.3%) under anaerobic condition. Multi-drug resistance was observed in 23.4% and 25% of total aerobic and anaerobic isolates respectively. Resistance to fluoroquinolones was lost post curing in Staphylococcusspp,Klebsiellaspp.
Conclusion: Females were more susceptible to sinusitis with recurrent episodes experienced compared to males. The presence of plasmid-mediated multidrug resistance genes
underscores the need for antibiograms, and rational antibiotics use insinusitis treatment.
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