WISTAR RATS TREATED

Anaemia is influenced by erythroferrone (ERFE), a regulator of hepcidin during stress erythropoiesis. Aluminium chloride induces anaemia through oxidative stress and impaired iron utilization. Icacina trichantha, a medicinal plant with reported haematopoietic and antioxidant effects, may modulate ERFE expression in anaemic conditions. The aim of this study is to determine the effect of aqueous leaf extract of Icacina trichantha on ERFE gene expression in aluminium chloride-induced anaemia in albino Wistar rats. A total of sixty (60) adult male albino Wistar rats were divided into six (6) groups; A, B, C, D, E and F representing control, aluminium chloride group, ferrous sulphate group, aluminium chloride + 100 mg/kg Icacina trichantha extract, aluminium chloride + 200 mg/kg Icacina trichantha extract and aluminium chloride + 400 mg/kg Icacina trichantha extract respectively. Haematological parameters, blood cell morphology and mRNA ERFE expression were determined using haematology autoanalyzer, manual method and polymerase chain reaction respectively. Data obtained were analyzed by the Statistical Package for Social Science (SPSS) software. The comparison of red blood cell parameters showed that red blood cell count (RBC) of groups C (7.65±0.21), D (8.18±0.06), E (7.84±0.31) and F (8.05±0.29) showed no significant difference when compared with groups A (8.09±0.12) and B (7.74±0.21) (p>0.05). Haemoglobin Concentration (g/dL) of groups C (15.06±0.36), D (15.5±0.22), E (15±0.59) and F (15.1±0.61) was not significantly different from groups A (15.33±0.24) and B (14.84±0.32) (p>0.05). Haematocrit (%) of groups C (44.46±0.99), D (45.1±0.73), E (44.32±1.46) and F (43.86±1.52) was not significantly different from groups A (44.88±0.78) and B (44.52±0.96) (p>0.05). Mean cell volume (MCV) of group F (54.64±0.96) was significantly lower than group C (58.22±0.49) (p<0.05), while no significant differences were observed in other groups. Mean cell haemoglobin (MCH) of group F (18.72±0.23) was also significantly lower when compared to group C (19.66±0.07) (p<0.05). Mean cell haemoglobin concentration (MCHC), RDW-SD, and RDW-CV showed no significant differences among the groups (p>0.05). Normochromatic and normocytic cells were moderately present in most groups (A, C, D, E), while Group B showed reduced levels with increased crenated cells. Group F demonstrated higher normocytic cell presence alongside mild crenation. Polychromatic cells were absent across all groups. Groups B showed significantly higher expression of RUNX1 when compared to groups A (p<0.05). Groups B showed significantly lower expression of ERFE when compared to groups A (p<0.05). Group C, D, E and F had significantly higher expression of ERFE when compared to group B (P<0.05). In conclusion, treatment with ferrous sulphate and varying doses of Icacina trichantha extract caused changes in red blood cell parameters and also improved ERFE expression, with the highest extract dose showing the greatest effect.