S.O. OLUBODUN

The experiment is aimed at carrying out a toxicological study on rats given different doses of both aqueous and methanol extract of Acalypha Wilkesiana and to determine the degree of toxicity on some organs of the rats fed with both aqueous and methanol extract of Acalypha Wilkesiana. A total of seventy five male albino rats were used for the whole of the experiments. For Acute toxicity study phase one of the studies alone was carried out. For sub-acute toxicity study: The rats were placed into 4 different groups and group 1 served as the control group that was fed with pellets and water, the other groups that’s group 2 to 4 was divided into 2 sub-groups (group 2A aqueous and 2B methanol), and was given different doses of plant extracts. For 28 days, to mark the end of our sub-acute toxicological study, liver and kidney function biomarkers, electrolytes, lipid profile assays were investigated. For the acute toxicity study, it was observed that there was no visible sign of toxicity or death observed in all
the animals administered with the aqueous and methanol extract of Acalypha Wilkesiana

This research examines the effect of ethanol leaf extract of Acalypha wilkisenia on malondialdehyde (MDA) and glutathione peroxidase (GPx) levels in wistar rats with 1,2 dimethylhydrazine(DMH) induced colon tumors. Colon cancer is a leading cause of cancer related mortality ,is often characterized by elevated oxidative stress markers such as MDA and altered antioxidant enzyme activities, including GPx. Alcalypha wilkisenia, a plant commonly known for its antioxidant properties, was evaluated for its potential therapeutic effects. Wistar rats were divided into six groups: Control, DMH treated only, Xeloda, DMH plus ethanol leaf extract of Alcalypha wilkisenia . Over the experimental period, MDA and GPx levels were measured using standard biochemical assays. Results indicated that DMH significantly increased MDAlevels and decreased GPx activity, indicative of oxidative stress and impaired antioxidant defense. Conversely administration of Acalypha wilkisenia extract to DMH treated rats resulted in a significant reduction in MDA levels and restoration of GPx activity , suggesting a protective effect against colon carcinogenesis through the modulation of oxidative stress markers, highlighting its potential as a complementary therapeutic agent in colon cancer management .Further studies are needed to elucidate the underlying mechanisms and confirm these effects in clinical settings