CALYX EXTRACT

ANTIBACTERIAL ACTIVITY OF Hibiscus Sabdariffa CALYX EXTRACT AGAINST SOME ENTERIC BACTERIAL ISOLATES

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Abstract
The emergence of antibiotic-resistant pathogens has garnered considerable focus on the antibacterial attributes of natural compounds in recent years. This study aims to evaluate the antibacterial efficacy of Hibiscus sabdariffa calyx extract against several enteric bacteria, specifically Escherichia coli, Salmonella typhi, Staphylococcus aureus, and Enterococcus faecalis. A phytochemical analysis of the Hibiscus sabdariffa calyx extract was conducted to identify and quantify bioactive compounds that may contribute to its antibacterial properties.This analysis identified secondary metabolites, such as tannins, flavonoids, and alkaloids,recognised for their antimicrobial properties. These compounds likely contribute significantly to the antibacterial effects observed in the hibiscus sabdariffa calyx extract. Furthermore, the antibacterial efficacy was assessed utilising recognised microbiological methodologies, including minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC),against a selection of enteric bacterial isolates. The results demonstrated a significant antibacterial effect linked to Hibiscus sabdariffa calyx extracts. Their inhibition of bacterial growth was dose-dependent, with ethanolic extracts demonstrating significantly greater efficacy than aqueous extracts
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