PERSEA AMERICANA

Malaria is an acute, life-threatening parasitic infection caused by protozoan parasites of the genus Plasmodium, posing a significant global health threat. The most virulent form that infects humans is Plasmodium falciparum. Current first-line treatments involve Artemisinin-based combination therapies (ACTs), but the increasing prevalence of antimalarial drug resistance constitutes a major impediment to global malaria control initiatives. Historically, traditional knowledge of indigenous plants has guided the discovery of effective antimalarials, such as quinine and artemisinin, underscoring the urgent need to explore plant-based medicines for alternative therapeutic strategies. Persea americana (avocado), a commercially valuable fruit tree, is used traditionally for malaria treatment. This study aimed to evaluate the methanolic extract of Persea americana seed for its antimalarial activity and acute toxicity level. The overall objective was to scientifically validate the plant’s use for anti-malarial therapy and suggest it as a promising source for new antimalarial compounds. Acute toxicity was assessed in six male Wistar strain mice (6–8 weeks old) using the limit test dose up and down procedure of the Organisation for Economic Cooperation and Development (OECD) guideline 423. Antimalarial activity was tested using the Peter’s 4-day Suppressive test in 20 male Wistar strain mice inoculated intraperitoneally with Chloroquine Sensitive Plasmodium berghei NK65 infected red blood cells. Extract doses of 100, 250, and 500 mg/kg b.wt. were administered orally and daily for four days. Control groups received normal saline vehicle, chloroquine (25 mg/kg b.wt.), or lithium chloride (10 mg/kg b.wt.). In the acute toxicity study (OECD guideline 423), following the administration of 2000 mg/kg b.wt. of the Persea americana seed methanol extract, no mice death was recorded, and no other signs of toxicity were observed during the 14-day period. Therefore, the extract was deemed safe for administration at 2000 mg/kg b.wt.. The findings suggest that the Persea americana seed methanol extract has a low acute toxicity profile, as demonstrated by the safety of administering 2000 mg/kg b.wt. in mice. The antimalarial activity of Persea americana recorded decrease in % Parasitemia in mice and the most significant decrease in the highest dose administered. This validates the traditional use of the plant sample and highlight the Persea americana seed as a promising resource for discovering new antimalarial compounds

Research has been ongoing on many plant materials, especially those discarded as waste, to exploit their nutritional and antinutritional properties. Generally, plant parts (seeds, leaves, bark, fruits, stems) contain bioactive agents with medicinal properties but require proper assessment. Avocado (P. americana) seeds are often discarded after taking the pulp of the fruit. Until recent times, Researchers have identified a wealth of therapeutic compounds in the husk of avocado seeds which are normally discarded. The present study was aimed at determining the level of anti-nutrients and possibly heavy metals in the seed of Avocado. The seeds were removed from the pulp, washed, dried, cut into smaller pieces and allow to air dry completely and then pulverised. The seed powder of P. americana was analysed to determine its mineral contents and some antinutritional properties (oxalate and phytate). The quantitative analysis of these parameters showed that the anti-nutritive components are Phytate (1.07%) and oxalate (6.16%) respectively. The mineral analysis revealed zinc (3.415 mg/kg ±0.145), Lead (0.035 mg/kg ±0.005), iron (2.775 mg/kg ± 0.105), Nickel (0.115 mg/kg ±0.005), Copper(0.265 mg/kg ±0.015), Cobalt (0.14 mg/kg ±0.01), Chromium (0.08 mg/kg ±0) respectively. This study, therefore revealed that P. americana seeds have a low level of oxalate and phytate contents while there were low levels of some heavy metals as seen in the result. In conclusion, the seeds of P.
Americana were found to be a beneficial potential therapeutic agent.