TAIWO CONFIDENCE OGEDENGBE

The crude extract of Anthocleista djalonensis has been evaluated to have an effect on Benign Prostatic Hyperplasia. This study aims to evaluate the effect of chromatographic fractions of the plant on Benign Prostatic Hyperplasia. The aqueous extract of the leaves of the plant obtained, was subjected to Vacuum Liquid Chromatography, and the Ethyl acetate - Methanol Fraction and the 100% Methanol fraction (MEL) were bulked together and concentrated. Column Chromatography was performed on MEL; thereafter, thin-layer chromatography was performed, which showed that Fractions 2 and 3 contained similar constituents, hence were bulked together to afford Fraction A Nineteen male Wistar rats were divided into 5 groups and were used for this study. Group 1 was the normal control group, animals in this group were neither induced nor treated. Group 2 was the negative control group and were induced with BPH using 5 mg/kg testosterone acetate (dissolved in coconut oil) once daily via the subcutaneous route. Animals in group 3 and 4 were administered 25 mg/kg and 50 mg/kg of Fraction A respectively using the orogastric tube. Group 5 was the positive control group and were also administered 4 mg/kg finasteride (The reference) once daily using the orogastric tube after having dissolved it in a vehicle. This administration was done across 14 days. At the end of the 14th day, the rats were sacrificed, the prostate was harvested, and blood was collected. Histological evaluation of the prostate was done. Serum hormonal profile of testosterone and prostate-specific antigen was analysed using the Enzyme-Linked Immunosorbent Assay technique (ELISA). Also, prostate weight and volume was determined from the harvested prostate. Results showed that doses of fraction A impacted prostatic indices, and histological studies showed reduced hyperplasia. These advocates that the leave extract of Anthocleista djalonensis possess Anti-BPH activity supporting ethnobotanical studies. However, further studies have to be carried out in order to ascertain its specific mechanism of action as well as toxicity
studies in order to ascertain its safety