God's greatness Osas ODION

D3 Organic is a supplement composed of various plant materials, including Desmodium gangeticum, Eclipta alba, Garcinia kola, Ocimum sanctum, Curcuma longa, and Tetracarpidium conophorum. The aim was to determine the antitussive and expectorant properties of the D3 Organic® supplement . In an antitussive study, 25 mice were allotted to 5 groups of 5 mice in each group. Group 1 is the control, group 2 is dihydrocodeine (25 mg/kg), and groups 3–5 are D3 Organic® supplement extract at 25, 50 and 100 mg/kg, respectively. One hour after oral drug administration, all the mice were exposed to 25% NH₄OH ( ammonium hydroxide) to induce cough. In the expectorant, 30 mice were allotted in 6 groups of 5 mice in each group. Group 1 is the control, group 2 is bromohexane (15 mg/kg), and groups 3–5 are D3 Organic supplement extract at 25, 50 and 100 mg/kg, respectively. Group 4 was given Sodium Chromoglycate (NaCr) at a dose of 50 mg/kg. All treatments were administered orally for five days, except Sodium Chromoglycate. In the last 30 minutes after Intraperitoneal administration of sodium chromoglycate, 5 mg/kg of ammonium chloride was given to each of the mice orally. Phenol red at a dosage of 500 mg/kg was administered intraperitoneally (IP) 30 minutes after the other drug administration. The mice were sacrificed, and the tracheal length of 2 cm was removed. Each piece of trachea was kept in 2 mL of normal saline for 30 minutes, and 0.1 mL of 1 M NaOH solution was added to the saline, and the absorbance at 460 nm was measured using a spectrophotometer.D3 Organic® supplement (25, 50 and 100 mg/kg) and dihydrocodeine (25 mg/kg) reduced the number of cough bouts when compared to the control (p<0.05) for ammonia induced cough in mice.D3 Organic® supplement (25, 50 and 100 mg/kg) and bromohexane (15 mg/kg) increased phenol red secretions when compared to control (p<0.05). The experimental results demonstrate that the aqueous leaf extract of D3 Organic® possesses significant antitussive and expectorant properties,