MICROBIAL AND PATHOLOGICAL SCREENING OF Lactobacillus casei IN ENHANCING IMMUNITY AGAINST Shigella flexneri IN WISTAR ALBINO RATS
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Abstract
Shigella flexneri, a leading cause of bacillary dysentery, induces severe gastrointestinal inflammation, disrupting gut homeostasis. This study evaluates the potential of Lactobacillus
casei as probiotics to enhance immune responses in Wistar albino rats against Shigella flexneri infection. Forty healthy Wistar rats were divided into five groups: Control, challenged (Shigella flexneri-infected), Probiotic (Lactobacillus casei-treated), Prophylactic (pre-treated with Lactobacillus casei before infection), and Antibiotic (ciprofloxacin-treated). Stool samples were collected from rats prior to induction with shigella flexneri and Lactobacillus casei, after induction with Shigella flexneri, and antibiotic treatment. Approximately 1g of stool was homogenized in 9 mL of sterile PBS using a vortex mixer to ensure thorough dispersion. A tenfold serial dilution was performed by transferring 1 mL of the stool homogenate into 9 mL of sterile PBS, creating dilutions from 10' to 10-6. From the 1 dilution, 0.1 mL was plated onto Nutrient agar plates and incubated at 37°C for 24hrs
casei as probiotics to enhance immune responses in Wistar albino rats against Shigella flexneri infection. Forty healthy Wistar rats were divided into five groups: Control, challenged (Shigella flexneri-infected), Probiotic (Lactobacillus casei-treated), Prophylactic (pre-treated with Lactobacillus casei before infection), and Antibiotic (ciprofloxacin-treated). Stool samples were collected from rats prior to induction with shigella flexneri and Lactobacillus casei, after induction with Shigella flexneri, and antibiotic treatment. Approximately 1g of stool was homogenized in 9 mL of sterile PBS using a vortex mixer to ensure thorough dispersion. A tenfold serial dilution was performed by transferring 1 mL of the stool homogenate into 9 mL of sterile PBS, creating dilutions from 10' to 10-6. From the 1 dilution, 0.1 mL was plated onto Nutrient agar plates and incubated at 37°C for 24hrs
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