Chidera Princessa OKUTE

Nature has provided humans with medicine, shelter, food, clothing, fragrances, and transportation throughout history. The D3 organic® supplement's antidepressant and anxiolytic properties are assessed. Antidepressants and anxiolytics were tested in the forced swim, tail suspension, and elevated plus maze. Twenty-five 20–30 gram mice were divided into five groups of five. Group 1 received 10 ml/kg distilled water, Groups 2, 3, and 4 received 50, 100, and 200 mg/kg D3 organic® supplement extract, and Group 5 received 20 mg/kg oral fluoxetine. After receiving D3 and fluoxetine, mice were placed in an unbreakable transparent cylinder filled with water at 25°C for one hour. Animal immobility was measured after 5 minutes of swimming. Twenty 20–30 gram mice were randomly assigned to five four-animal groups. Group 1 received distilled water (10 ml/kg), groups 2–4 D3 organic® supplement extract (50, 100, and 200 mg/kg), and group 5 diazepam. The animals spent five minutes in the central maze an hour after receiving D3 organic® supplement extracts and diazepam. The number of entries and open arms time were recorded. In the forced swimming and tail suspension test, D3 organic® supplement extract (100 and 200 mg/kg) and fluoxetine (20 mg/kg) reduced immobility time compared to the control (p<0.05). Diazepam (10 mg/kg) and D3 organic® supplement extract (100 and 200 mg/kg) increased time spent and percentage time spent in the open arm compared to the control (p<0.05). D3 Organic® Supplement has anxiolytic and antidepressant properties.