Chiamaka Juliet EDOZIEM

EFFECT OF INCUBATION PERIOD ON THE ANTIBACTERIAL ACTIVITY OF MAGNESIUM CHLORIDE NANOPARTICLES ON BACTERIA ISOLATED FROM A DISEASED Manihot esculenta

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Abstract
Nanotechnology is an emerging field of science that includes synthesis and development of various nano-materials. The aim of this study was to determine the effects of incubation period on the antimicrobial activities of magnesium chloride nanoparticles on bacteria isolated from a diseased Manihot esculenta leaf. Three bacteria were isolated from the diseased Manihot esculenta leaf obtained from three different farms. The antibacterial activities of Moringa oleifera and aqueous extract were performed using agar well diffusion method. From the results, no activity was recorded against the test isolates (0.00+0.00 cm) but this was significantly different from the control which showed zone of inhibition ranging from 2.60+ 0.10 cm for Enterobacter Ludwigii GM7 to 3.20+0.10cm for Erwinia spp after incubation periods of 24hrs and 48hrs respectively. The result of this study reveals that magnesium chloride nanoparticles (at all concentrations) had no effect on the pathogens. Further studies should be however conducted to unravel the reasons behind this resistance by the test isolates against synthesized MgONp.
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