ANTIOXIDANT EFFECT OF SEED EXTRACT OF Persea americana IN BENIGN PROSTATE HYPERPLASIA RATS AND LC-MS PROFILING OF IT'S METABOLITES
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Benign prostate hyperplasia is a disease of ageing men. Oxidative stress is a promoter of the ageing process. This study evaluated the effect of the ethanol seed extract of Persea Americana (PAE) on antioxidant and lipid per oxidation status of male rats induced with benign prostate hyperplasia and spectroscopic analysis of the extract. Five groups of five rats each were induced
with benign prostate hyperplasia by the subcutaneous administration of testosterone (3mg/kg). Group 1 rats (negative control) received 10 ml/kg of distilled water. Group 2 animals (standard control) were treated with finasteride (4mg /kg). Groups 3, 4 and 5 received 100, 200 and 400 mg/kg dose of PAE respectively. Groups 6 animals (normal control) were neither induced nor
treated. All administration was daily for 28 days by oral gavage. Rats were sacrificed on the 29 th day, blood obtained, and serum enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (Gsr) and glutathione peroxidase (GPx) were measured with Elisa assay test kits. Malondialdehyde concentration was equally measured. High-Performance Liquid
Chromatography-Mass Spectrometry (HPLC-MS) was used to identify compounds present in PAE. The extract significantly (P<0.0001) increased the activities of SOD, CAT, GPx and Grs compared to the negative control at the dose of 400 mg/kg while MDA concentration was significantly reduced. Digoxin, tetramethylquercetin and abscisic acid were identified in PAE
with benign prostate hyperplasia by the subcutaneous administration of testosterone (3mg/kg). Group 1 rats (negative control) received 10 ml/kg of distilled water. Group 2 animals (standard control) were treated with finasteride (4mg /kg). Groups 3, 4 and 5 received 100, 200 and 400 mg/kg dose of PAE respectively. Groups 6 animals (normal control) were neither induced nor
treated. All administration was daily for 28 days by oral gavage. Rats were sacrificed on the 29 th day, blood obtained, and serum enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (Gsr) and glutathione peroxidase (GPx) were measured with Elisa assay test kits. Malondialdehyde concentration was equally measured. High-Performance Liquid
Chromatography-Mass Spectrometry (HPLC-MS) was used to identify compounds present in PAE. The extract significantly (P<0.0001) increased the activities of SOD, CAT, GPx and Grs compared to the negative control at the dose of 400 mg/kg while MDA concentration was significantly reduced. Digoxin, tetramethylquercetin and abscisic acid were identified in PAE
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