COMPARATIVE STUDY ON THE FLAVONOID CONTENT AND HYDROGEN PEROXIDE SCAVENGING ACTIVITY OF Kigelia africana METHANOL AND FLAVONOID-RICH EXTRACTS AND THEIR ETHYLACETATE FRACTIONS
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Abstract
This study investigated different extraction methods and antioxidant properties of Kigelia africana through the analysis of extract yields, total flavonoid content, and hydrogen peroxide scavenging activity. Four extraction methods were evaluated: methanol crude extract (ME), flavonoid-rich extract (FRE), methanol extract-ethyl acetate fraction (ME EAA), and flavonoidrich extract-ethyl acetate (FRE EAA). The FRE EAA showed the highest yield (0.2173), while FRE demonstrated the lowest yield (0.016). Total flavonoid content analysis revealed highest concentrations in the flavonoid-rich extract (185.6 ± 4.2 mg QE/g), followed by ethyl acetate fraction (142.3 ± 3.8 mg QE/g), and methanol extract (98.7 ± 2.9 mg QE/g). All extracts
exhibited concentration-dependent hydrogen peroxide scavenging activity, with IC50 values ranging from 7.508 ± 0.4 mg/mL (ME) to 7.644 ± 0.2 mg/mL (FRE). These findings suggest that while different extraction methods significantly affect yield and flavonoid content, all extracts
demonstrate comparable antioxidant activity, with the flavonoid-rich extract showing particularly promising results for potential therapeutic applications.
exhibited concentration-dependent hydrogen peroxide scavenging activity, with IC50 values ranging from 7.508 ± 0.4 mg/mL (ME) to 7.644 ± 0.2 mg/mL (FRE). These findings suggest that while different extraction methods significantly affect yield and flavonoid content, all extracts
demonstrate comparable antioxidant activity, with the flavonoid-rich extract showing particularly promising results for potential therapeutic applications.
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