Aguebor-Ogie Nogiowman Bobby

TOTAL PHENOLAND FLAVONOID CONTENT OF DRIED Gossypium (COTTON) SEEDS

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Abstract
Cotton (Gossypium spp.) seeds, a primary by-product of the global cotton industry, are often underutilized but represent a potential source of valuable bioactive compounds. This study was designed to quantify the total phenolic content (TPC) and total flavonoid content (TFC) of dried cotton seeds to evaluate their potential for value-added applications. An aqueous extract was prepared from dried, powdered seeds, and the presence of phenols and flavonoids was confirmed using standard qualitative phytochemical screening. Total phenolic content was determined spectrophotometrically using the Folin-Ciocalteu method with tannic acid as the standard, while total flavonoid content was measured via the aluminum chloride colorimetric assay using quercetin as the standard. The quantitative analysis revealed a substantial total phenolic content of 82.35 ± 0.40 g of tannic acid equivalents per kilogram (g TAE/kg) of the dried sample. The total flavonoid content was also significant, measured at 9.15 ± 1.24 g of quercetin equivalents per kilogram (g QE/kg). These findings demonstrate that dried cotton seed is a rich source of natural phenolic antioxidants, highlighting its potential for valorization as a value-added ingredient in functional food, nutraceutical, and animal feed industries and promoting a more sustainable circular economy in cotton production.
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ACID VALUE, % FREE FATTY ACID AND PEROXIDE VALUE OF Elaeis guineensis (PALM KERNEL) OIL, Gossypium hirsutum (COTTON SEED) OILAND Glycine max (SOYA BEAN) OIL

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Abstract
The quality of edible oils is a critical factor for consumer health, yet deterioration through hydrolysis and oxidation can lead to rancidity and the formation of toxic compounds. This study aimed to evaluate and compare the quality of commercially available Glycine max (Soya Bean Oil), Elaeis guineensis (Palm Kernel Oil), and Gossypium hirsutum (Cotton Seed Oil) by determining key degradation indices. Oils were extracted from seeds sourced from a local market using the Soxhlet method. The acid value (AV), percentage free fatty acid (%FFA), and peroxide value (PV) were determined in triplicate for each oil sample using standard titrimetric methods (AOCS). The results revealed profound disparities in quality among the oils. Soya Bean Oil exhibited acceptable quality, characterized by a low acid value (3.35 ± 0.03 mg KOH/g), low %FFA (1.68 ± 0.02%), and a moderate peroxide value (13.07 ± 0.92 meq/kg). In stark contrast, Palm Kernel Oil and Cotton Seed Oil were of exceptionally poor quality, displaying extremely high acid values (23.30 ± 3.02 and 26.13 ± 2.75 mg KOH/g, respectively) and alarmingly high peroxide values (99.59 ± 0.80 and 107.49 ± 3.78 meq/kg, respectively). These findings indicate that while the SBO was of acceptable quality, the palm kernel oil and cotton seed oil samples exhibited severe hydrolytic and oxidative rancidity, far exceeding international safety standards and rendering them unsuitable for consumption. This study highlighted a critical public health issue regarding the quality of some oils available in local markets and underscores the urgent need for stricter quality control measures throughout the supply chain to mitigate health risks associated with consuming degraded oils.
co-supervisor