ISOLATION AND IDENTIFICATION OF BACTERIA FROM URINE OF MALE UNDERGRADUATES IN UNIVERSITY OF BENIN
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This present study focused on isolation and identification of bacteria isolated from urine sample of undergraduate students at the University of Benin, Benin City. A total of fifteen (15) urine samples were collected and transported to the laboratory for bacteriological analysis. The total bacteria count ranged from 6.2 × 10 5 CFU/ml - 8.0 × 10
5 CFU/ml. Bacterial species isolated includes Escherichia coli, Staphylococcus aureus, Staphylococcus saprophyticus and Proteus mirabilis. E. coli was the most predominant isolate accounting for about (55%) followed by S. aureus (25%), S. saprohyticus (15%) and P. mirabilis (5%). The antibiotic susceptibility to antibacterial agent revealed that of S. aureus all the isolates were (100%) resistant to Zinnacef and Cephalexin while they were (100%) sensitive to ciprofloxacin. S. saprohyticus 15(100%) were resistant to cephalexin and (100%) sensitive to ciprofloxacin. E. coli and P. mirabilis susceptibility test revealed that 5(5%) of P. mirabilis showed resistance to Cephalexin and Septrin and was sensitive to Ciprofloxacin, Amoxicillin and Augmentin. however, 55(100%) of E. coli were resistant to cephalexin, moderate sensitivity to septrin and (100%) sensitivity to
ciprofloxacin. The isolates were also tested for their ability to produce gelatinize. All bacterial isolates produced gelatinize. The ability of the bacterial isolates obtained to produce gelatinize is of a serious medical importance as this may have contributed to the multi-drug resistance of the isolates and as such, further research should be carried out to discover new antibiotics effective against these organisms.
5 CFU/ml. Bacterial species isolated includes Escherichia coli, Staphylococcus aureus, Staphylococcus saprophyticus and Proteus mirabilis. E. coli was the most predominant isolate accounting for about (55%) followed by S. aureus (25%), S. saprohyticus (15%) and P. mirabilis (5%). The antibiotic susceptibility to antibacterial agent revealed that of S. aureus all the isolates were (100%) resistant to Zinnacef and Cephalexin while they were (100%) sensitive to ciprofloxacin. S. saprohyticus 15(100%) were resistant to cephalexin and (100%) sensitive to ciprofloxacin. E. coli and P. mirabilis susceptibility test revealed that 5(5%) of P. mirabilis showed resistance to Cephalexin and Septrin and was sensitive to Ciprofloxacin, Amoxicillin and Augmentin. however, 55(100%) of E. coli were resistant to cephalexin, moderate sensitivity to septrin and (100%) sensitivity to
ciprofloxacin. The isolates were also tested for their ability to produce gelatinize. All bacterial isolates produced gelatinize. The ability of the bacterial isolates obtained to produce gelatinize is of a serious medical importance as this may have contributed to the multi-drug resistance of the isolates and as such, further research should be carried out to discover new antibiotics effective against these organisms.
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