STEM BARK

Medicinal plants are plants that generally contain constituents that have been found useful for the treatment and management of both animal and human diseases. Diabetes mellitus is a metabolic disorder resulting from a defect in insulin secretion, insulin action or both. This present work determined the ameliorative effect of the histopathological changes in Streptozotocin-Induced diabetic rats. Male wistar rats were purchased of age (16weeks), kept in clean and serene cages and left to acclimatize for two weeks and were fed with normal poultry feed before inducing them with Streptozotocin according to their weight and were divided into 7 groups and were administered treatment using Metformin, crude extract, ethyl acetate of 200mg and 400mg respectively. From the histopathological studies of the liver, it showed that the control group had hepatocytes and central vein organized, orderly and patterns are regular. The negative control group showed periportal hepatitis, inflammation of the hepatocytes and zonal necrosis while the group treated using Metformin showed a little improvement but there was still portal hepatitis and necrosis. When 200mg of the crude extract was administered, necrosis was reduced, inflammation persisted but mild. 400mg of the crude extract ameliorated the disease, no inflammation, no congestion, no necrosis, fine hepatocytes, Sinosoids were seen properly and Kupffer cells were activated. 200mg ethyl acetate tried in ameliorating the effects of the Streptozotocin damage. Small inflammation was observed, small congestion was observed , necrosis were really reduced and there was Kupffer cell activation too. 400mg ethyl acetate was administered and the inflammation though mild became more obvious compared to the 200mg of ethyl acetate which did a better job at ameliorating the effects of the Streptozotocin. The bile ducts were obvious and no congestion was observed.

A wide array of ethno-medicinal values has been attributed to the different parts of Annona muricata, and indigenous communities in Africa and South America extensively use this plant to augment conventional drugs. Acute and subchronic toxicity studies were carried out on the stem bark extract of Annona muricata Linn. The aim of this study was to determine the effect of A. muricata on hematological parameters in wistar albino rats. A total of Sixty (60) male albino rats were divided into 12 groups of five (5) rats each. They were given grower pellets and water. Groups one (control), two, three, four, five, six were administered 0, 200, 500, 1000, 3000 and 5000 mg/kg body weight of the extract per day respectively for a period of 28days.Weekly measurements of body weight were carried out. After 30 days, the rats were subjected to overnight fast after which they were sacrificed, with their
blood and organ samples collected into sterile containers with anticoagulant. Hematological profiles showed increased levels of red blood cells and its related indices for both methanolic and aqueous extract of Annona muricta. The investigation into the sub-chronic toxicity of methanol and aqueous extract of the stem bark of Annona muricata revealed no significant effect on the immunity of wistar albino rats.