ACUTETOXICITY OFETHANOLROOT EXTRACT OFMoringa oleiferaLAM.INSWISSMICE
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Abstract
Moringa oleifera lam is a tree species belonging to the family moringaceae. It is widely appreciated for its ornamental and medicinal attribute. This study was carried out to determine the acute toxicity of M. oleifera in Swiss mice to determine the LD50 and observe any physiological and behavioral changes. Fresh roots of the M. oleifera were prepared by soaking dried
powdered roots of the plant species in absolute ethanol for 72 hours. After filtering and concentrating, various doses of the extract were prepared for administration. Twenty-eight adult male Swiss mice weighing between 30-35 g were used. In phase I, twelve mice were randomly assigned into four groups (I – IV) of three mice each. Groups I - III received single oral
doses of 10, 100, 1000 mg/kg respectively of M. oleifera ethanol root extract, and the control (Group IV) received distilled water. After 24 hours observation, Phase II experiment was conducted with three mice per group receiving single doses of 1600, 2900, 5000 and 10000 mg/kg of the extract respectively, control was given distilled water. They were observed for 14 days after administration. There was no mortality in both phases and no observable alterations in the mice treated with the M. oleifera ethanol root extract. Even at a high dosage of 10000 mg/kg, no observable alterations such as Writhing, Pilo-erection, Jerking, Lacrimation, Salivation, Hemorrhage and Nausea were observed. The lethal dosage (LD50) of the ethanol root extract was determined to be >10000 mg/kg, which implies a relatively high safety limit.
powdered roots of the plant species in absolute ethanol for 72 hours. After filtering and concentrating, various doses of the extract were prepared for administration. Twenty-eight adult male Swiss mice weighing between 30-35 g were used. In phase I, twelve mice were randomly assigned into four groups (I – IV) of three mice each. Groups I - III received single oral
doses of 10, 100, 1000 mg/kg respectively of M. oleifera ethanol root extract, and the control (Group IV) received distilled water. After 24 hours observation, Phase II experiment was conducted with three mice per group receiving single doses of 1600, 2900, 5000 and 10000 mg/kg of the extract respectively, control was given distilled water. They were observed for 14 days after administration. There was no mortality in both phases and no observable alterations in the mice treated with the M. oleifera ethanol root extract. Even at a high dosage of 10000 mg/kg, no observable alterations such as Writhing, Pilo-erection, Jerking, Lacrimation, Salivation, Hemorrhage and Nausea were observed. The lethal dosage (LD50) of the ethanol root extract was determined to be >10000 mg/kg, which implies a relatively high safety limit.
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