Antidiabetic and Antioxidant effect of Phyllantus amarus in TYPE 2 DIABETES in Drosophilia melanogaster
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Abstract
Diabetes is a chronic metabolic disorder defined by increased levels of circulating blood sugar (hyperglycemia) caused by abnormal insulin secretion and/or signaling. Diabetes mellitus is divided into type 1 and type 2, a division that reflects the cause
of the metabolic Dysfunction. There is increasing evidence that complications related to diabetes are associated with oxidative stress, induced by the generation of free radicals. The plant, Phyllanthus amarus have antidiabetic and antioxidant
properties. The fruit fly, Drosophila melanogaster is a highly suitable system to model type 2 diabetes because mechanisms of glucose homeostasis are conserved between flies and humans, and it allows for substantial ease of experimental and
genetic manipulation in comparison to rodent models. This study was done to find out the antidiabetic and antioxidant effect of P. Amarus in Type 2 diabetic D.melanogaster flies. Both genders of D.melanogaster flies (Harwich strain) of 1-3 days old were divided into four groups with each group containing 50 flies. Group 1 served as control and the flies were treated with basal diet. Group 2 flies were fed with 30% high sucrose diet. Group 3 flies were fed with 30% high sucrose diet and 40Mm P. amarus. Group 4 flies were fed with 30% high sucrose diet and 40mM silymarin. The flies were monitored under a natural photoperiod of about 12 hours light and 12 hours dark daily for a period of 21 days and was replicated 5 times. The flies were monitored daily and the survival was done.
of the metabolic Dysfunction. There is increasing evidence that complications related to diabetes are associated with oxidative stress, induced by the generation of free radicals. The plant, Phyllanthus amarus have antidiabetic and antioxidant
properties. The fruit fly, Drosophila melanogaster is a highly suitable system to model type 2 diabetes because mechanisms of glucose homeostasis are conserved between flies and humans, and it allows for substantial ease of experimental and
genetic manipulation in comparison to rodent models. This study was done to find out the antidiabetic and antioxidant effect of P. Amarus in Type 2 diabetic D.melanogaster flies. Both genders of D.melanogaster flies (Harwich strain) of 1-3 days old were divided into four groups with each group containing 50 flies. Group 1 served as control and the flies were treated with basal diet. Group 2 flies were fed with 30% high sucrose diet. Group 3 flies were fed with 30% high sucrose diet and 40Mm P. amarus. Group 4 flies were fed with 30% high sucrose diet and 40mM silymarin. The flies were monitored under a natural photoperiod of about 12 hours light and 12 hours dark daily for a period of 21 days and was replicated 5 times. The flies were monitored daily and the survival was done.
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