ETHYL ACETATE EXTRACT

Multi-Drug Resistance developed by pathogens to most of the antibiotics present in the world today has led to an increase in the interest on plant derived compounds as substitute. In this study the antimicrobial activity of ethyl acetate extract of hrysophyllum albidum cotyledon was determined, as well as the phytochemical constituents, proximate parameters and chemical constituents of C. albidum cotyledon. The antimicrobial activity of the seed extract was evaluated against clinical isolates (Stapyhlococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, Klebsiella spp, Aspergillus nigans) using agar-well diffusion method, Proximate analysis and phytochemical studies were carried out using standard method as described by Association of Official Analytical Chemist(AOAC). A two phase (liquid:oil) brown coloured extract with a yield of 2.530% was
obtained. The extract inhibited the growth of all test bacteria with an inhibition zone diameter (IZD) of 25mm – 20.5mm. The phytochemical result revealed the presence of alkaloids, phenolic, flavonoid, tannins, terpenoids, carbohydrate, proteins, reducing sugars, phytosterol and amino acids.