MALE UNDERGRADUATE’S STUDENTS

The scalp hair is composed of soft tissue layers that cover the cranium. This study focused on isolation, identification and antibiogram of bacteria isolates from scalp hair. With the aid of a sterile swab sticks, nine scalp hair of undergraduates’ students were swabbed and transported to the laboratory for bacteriological analysis. All the samples were analyzed within 24hrs of collection. Collected swabbed stick was submerged in a sterile test tubes label A – I contained Nutrient broth and incubated for 2hrs. 1ml was plated from each test tube. It was then incubated at 37 0 C for 24hours. The result of the bacteria isolated include; Staphylococcus epidermis 6 (15%), Staphylococcus aureus 6 (15%), Pseudomonas sp. 2(5%), Bacillus sp. 18(45%), Streptococcus spp. 5(12.5%) and Micrococcus spp. 3(7.5%). Haemolysin test on the strains of Staphylococcus auraeus Streptococcus sp. Staphylococcus epidermis, Micrococcus spp. and Psuedomonas sp. revealed they had β hemolytic activity and only strains of Bacillus spp. showed α hemolytic activity. While the gelatin test showed that all isolates produce gelatinase enzyme which breakdown gelatin. The antibiogram results revealed that All isolates were highly sensitive to ciprofloxacin, Ofloxacin and clindamycin 40(100%). Ciprofloxacin, Ofloxacin and clindamycin was proved to be the most effective against Gram positive and Gram-negative isolates studied in this work. Contamination of scalp hair from this research, could be from poor hand hygiene and environmental hygiene.