Hydrogen peroxide scavenging Superoxide scavenging activity Thiobarbituric acid reactive substances

In vitro antioxidant activities were carried out on the ethanol extracts of the stem bark of Anthocleista Djalonensis, The methods used were standard procedures for assessment of its antioxidant properties using DPPH scavenging activity, hydrogen peroxide scavenging assay, thiobarbituricacid reactive assay, superoxide scavenging activity and ferric reducing-antioxidants power with their standard as Ascorbic acid. The stem washed clean of sand after which it was air-dried at room temperature and ground into fine powder. The powdered sample (168g) was extracted with 4.9L of Ethanol by maceration for 72 hrs with regular stirring, The mixture was filtered using muslin cloth and concentrated using rotary evaporator and subjected to freeze drying to obtain powdered form. The result of 1,1-diphenol-2picrylhydrazyl (DPPH) radical scavenging activity of ethanol extract shows that the scavenging activity of DPPH was significantly higher (P>0.05) than the standard used(Ascorbic acid),it possesses Antioxidant activities. In the Thiobarbituricacid reactive substance (TBARs) assay, ethanol extract of A.djalonensis observed to be at bar with the standard used(Ascorbic acid). Although it was highest in the standard used. Since the A.djalonensis has been suspected to have antioxidant activities which helps in relieving stress and reducing organic species that enhances damage to the systems of the body, hence the experiment. In conclusion, from the results gotten A.djalonensis possesses good antioxidant properties, since it can be found naturally, it's use should be encouraged to help scavenge radicals and manage stress.