MICROPLASTIC CONCENTRATIONS IN CAT FISH (Clarias gariepinus) AND NILE TILAPIA FISH (Oreochromis niloticus) SPECIES FROM OGBA RIVER, BENIN CITY, NIGERIA
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Abstract
Microplastics have been recognized worldwide for their toxic effects in fish, man and wildlife. The Ogba River in Benin City, Nigeria, has been reported to be contaminated to varied levels on Microplastic concentrations as a result of anthropogenic impact. This preliminary study was conducted to determine the levels of Microplastic concentrations in Cat fish (Clarias
gariepinus) and Tilapia fish (Oreochromis niloticus) from Ogba river, Benin city, Nigeria. Microplastic is a minute particle of chemical pollutant in marine environment and classified as less than 5 mm size. The microplastics could not degrade for long years and they are ingested, incorporated, and accumulated in tissues of living organisms. The existence of
microplastics in living organisms is influenced by the interaction of biological and non- biological factors and ecological security, although the exact mechanism is unclear. Fish samples was collected using a fishing net or scoop net while operating a dug-out canoe with the assistance of local fishermen, samples was placed in labelled zip-lock bags and conveyed to the laboratory in an ice box. Samples was rinsed thoroughly with running water. The cleaned fish sample was placed in 10% KOH solution and the beaker was covered. The beaker was safely stored away for a period of 14-21 days, the samples was left to digest in
closed vials overnight at 60°C in an oven. The digested sample was purified using wet 30% H2O2. and sieved through two sieves (1 mm and 38 mm), or 5um to ensure the capturing of microplastics of the smallest sizes. The filter was rinsed into a glass petri-dish using pure water and was subjected to a temperature of a 100°C for 12hrs in an oven.
gariepinus) and Tilapia fish (Oreochromis niloticus) from Ogba river, Benin city, Nigeria. Microplastic is a minute particle of chemical pollutant in marine environment and classified as less than 5 mm size. The microplastics could not degrade for long years and they are ingested, incorporated, and accumulated in tissues of living organisms. The existence of
microplastics in living organisms is influenced by the interaction of biological and non- biological factors and ecological security, although the exact mechanism is unclear. Fish samples was collected using a fishing net or scoop net while operating a dug-out canoe with the assistance of local fishermen, samples was placed in labelled zip-lock bags and conveyed to the laboratory in an ice box. Samples was rinsed thoroughly with running water. The cleaned fish sample was placed in 10% KOH solution and the beaker was covered. The beaker was safely stored away for a period of 14-21 days, the samples was left to digest in
closed vials overnight at 60°C in an oven. The digested sample was purified using wet 30% H2O2. and sieved through two sieves (1 mm and 38 mm), or 5um to ensure the capturing of microplastics of the smallest sizes. The filter was rinsed into a glass petri-dish using pure water and was subjected to a temperature of a 100°C for 12hrs in an oven.
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