E. O. IGBINOSA

Wastewater generated from restaurants often contains a mixture of organic matter and microbial contaminants that may pose environmental and public health risks. This research focuses on the detection and isolation of Escherichia coli (E. coli) from wastewater collected from selected restaurants within the University of Benin, Benin City, Nigeria. For this study, wastewater samples were obtained from three restaurants: Helena’s Kitchen, Home and Away, and Buka— during peak operation hours. The samples were collected aseptically and analysed using standard microbiological methods. The pour plate technique was employed for total heterotrophic bacterial counts, while selective media such as Eosin Methylene Blue (EMB) agar were used for the isolation of E. coli. Biochemical tests including indole, methyl red, citrate, urease, and triple sugar iron (TSI) were used to confirm the isolates. The results showed high microbial loads across all samples, with E. coli being consistently present, indicating faecal contamination of the wastewater. The identification of other bacterial species suggest contamination from multiple sources such as food residues, human handling, and the environment. The findings reveal poor wastewater management and hygiene practices in the studied restaurants. In conclusion, the consistent presence of E. coli in restaurant wastewater signifies potential health and environmental hazards within the University of Benin. It is therefore recommended that wastewater from restaurants be regularly monitored, and that adequate sanitation infrastructure and treatment systems be put in place to prevent contamination and safeguard public health.