Energy drinks Blue Bullet energy drink Mineral composition Sugar content analysis Heavy metal screening Toxicological assessment Food safety evaluation Spectrophotometric analysis Benedict’s test Fehling’s test Carbohydrates Phosphates

Energy drinks are popular for their energy-boosting effects, yet concerns about their composition and safety persist. This study aimed to evaluate the mineral, sugar, and heavy metal content of Blue Bullet energy drink, focusing on its impact on energy metabolism, mineral homeostasis, and toxicological risks. Triplicate samples of the drink was prepared for the qualitative analyses were conducted to detect the presence of carbohydrates, phosphates, and acidity and quantitative tests which included Benedict’s and Fehling’s solutions for sugar content, spectrophotometric analysis for minerals, and toxicological screening for heavy metals. Qualitative analyses confirmed significant carbohydrate presence, trace phosphates, and an acidic pH typical of energy drinks. Quantitative screening revealed carbohydrates (12 ± 3 μg/L), alcohol (16 ± 2 μg/L), phosphate (368 ± 1 μg/L), and CO2 (78 ± 11 μg/L). Mineral analysis identified calcium (24.47 ± 0.15 mg/kg), iron (0.93 ± 0.03 mg/kg), potassium (0.30 ± 0.00 mg/kg), magnesium (1.17 ± 0.03 mg/kg), and phosphorus (1.667 ± 0.002 mg/kg), all within FDA and WHO safety limits. Trace metals such as chromium (0.047 ± 0.004 mg/kg), manganese (0.047 ± 0.012 mg/kg), and zinc (0.083 ± 0.003 mg/kg) were present at safe levels. Toxicological screening showed arsenic (0.955 ± 0.004 μg/kg) and mercury (0.070 ± 0.001 μg/kg) below safety thresholds, with no detectable lead, cadmium, or copper. These findings highlight that Blue Bullet provides essential nutrients and adheres to safety standards, though regular quality control is recommended for consumer safety..