BACTERIOLOGICAL AND PHYSCIOCHEMICAL ASSESSMENT OF BOREHOLE WATER IN SELECTED SECONDARY SCHOOLS WITHIN UGBOWO, BENIN CITY, EDO STATE
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Abstract
This study examines the microbiological risks associated with several borehole water samples found in selected Secondary Schools in Ugbowo axis. There were four Schools where borehole water samples (n=4) were taken. For both psychochemical and microbiological studies, samples were taken to the lab. Physical-chemical investigations were performed to measure the concentrations of phosphate, nitrate, and sulfate as well as pH, suspended particles, salinity, total hardness, electrical conductivity, and turbidity. To identify the bacteria isolates found in the school water samples, bacterial examinations were carried out. Additionally, biochemical tests such those for catalase, indole, oxidase, citrate, KOH, and sugar mentation were carried out. Utilizing the Kirby-Bauer disk diffusion method, the antimicrobial susceptibility testing of the various isolates was examined. Results from the study revealed the range values of physicochemical parameters as, The physicochemical properties of the water samples frequently ranged in temperature between 25.40 and 25.70°C. They had turbidities that varied between 0.50 and 1.95 nephelometric units (NTU). The pH range of borehole water samples is 6.06 to 7.66. The ranges for electrical conductivity, salinity, and the amount of suspended particles were 13 to 61 S/cm, 6.33 to 30.00 g/kg, and 0.24 to 0.85 mg/ml, respectively. The bulk of heavy metals were not detectable, which is why the result was 0.00 Biochemical test results revealed the presence of bacteria such as. Proteus mirabilis, Proteus vulgaris, Salmonella enterica, and Klebsiella oxytocic, The results obtained for the antimicrobial test revealed that zone of inhibition exhibited by Gentamycin, Erythromycin, Clindamycin, Augmentin, Ciprofloxacin, Colistin and Tetracycline ranged from 7mm-13mm ( partial resistance was also observed), only partial resistance in Erythromycin, 4mm-5mm, Nil, 5mm-10mm, Nil and 3mm respectively. In order to x prevent water-borne illnesses that could endanger humans, it is crucial to conduct more research
on the microbiological safety of surface water bodies. xi
on the microbiological safety of surface water bodies. xi
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