Lactobacillus sp.

This study investigates the isolation and characterization of Lactobacillus sp.Isolated Trace Minerals to evaluate their capacity for bacteriocin production.Lactic acid bacteria (LAB), especially those of the Lactobacillus sp, are well known for producing antimicrobial substances called bacteriocins. These natural peptides effectively inhibit the growth of harmful and spoilage microorganisms. Probiotic supplements such as Trace Minerals contain diverse Lactobacillus strains with strong bacteriocin-producing potential, making them valuable for both health and industrial applications.The isolates morphological and biochemical Properties was perform to confirm their identity to be Lactobacillus sp. Bacteriocin synthesis was assessed using the agar well diffusion technique to determine antimicrobial activity against selected test organisms. The influence of hydrogen peroxide neutralization and proteolytic enzyme treatment was examined to confirm the stability and proteinaceous nature of the produced bacteriocin. In addition, high- performance liquid chromatography (HPLC) was employed to characterize the molecular structure of the compound identified in the bacteriocin. This study isolated and identified Lactobacillus species from Trace Mineral probiotics to assess their bacteriocin production and antimicrobial activity against Escherichia coli. The isolates were cultured in MRS media under anaerobic conditions and confirmed through biochemical tests. Antimicrobial activity was evaluated using the agar well diffusion method, while enzyme and hydrogen peroxide treatments verified the protein nature of the bacteriocin. HPLC analysis confirmed the purity and presence of active bacteriocin fractions. The isolates displayed typical Lactobacillus characteristics and showed strong inhibitory effects against E.coli, supporting their potential as natural antimicrobial agents.This study confirms that Lactobacillus sp from Trace Mineral probiotics produce effective bacteriocins with strong activity against E.coli. The results verified the protein nature and purity of the compound, indicating its potential as a natural and safe antimicrobial agent for health and industrial use.