S.O Nwokoro

PROXIMATE COMPOSITION AND AMINO ACID PROFILE OF SCENT LEAF (Ocimum gratissimum L.) PROTEIN CONCENTRATE

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Abstract
This research work was conducted to evaluate the proximate composition and amino acid profile of scent leaf protein concentrate and scent leaf meal. Wet milling method was used for the extraction of the scent leaf protein concentrate. A grinder was used in the processing of the scent leaf meal. The amino acid profile of the scent leaf was determined using high performance liquid chromatography (HPLC). The Proximate analysis was carried out in triplicate with different procedures for moisture content determination, crude protein determination, crude fibre determination, ash determination, ether extract determination, and nitrogen free extract determination. From the result of the proximate analysis, the scent leaf meal contained a significant portion of crude protein, but the crude protein content of the scent leaf protein concentrate was significantly higher (p<0.05). It
was also observed that the crude fibre content of scent leaf meal was significantly higher (p<0.05) than that of scent leaf protein concentrate. It was also observed that there is more crude fat in scent leaf protein concentrate when compared to scent leaf meal (p<0.05). The scent leaf protein concentrate also has more ash content when compared to the scent leaf meal. From the result of the amino acid profile, it was observed that boththe scent leaf protein concentrate and the scent leaf meal have 18 amino acids, including essential amino acids, with scent leaf protein concentrate being higher in lysine and methionine than that of scent leaf meal. This study shows that scent leaf meal and scent leaf protein concentrate can be incorporated into animal and human diet.
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co-supervisor

DEVELOPMENT OF FEED GRADES USING SWEET POTATO (ipomoea batata [L.] LAM) LEAF MEAL AND TUBER PEELS

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Abstract
This study was conducted to examine the development of feed grades (25%, 50%, 70%) using sweet potato (Ipomoea batata L.) leaf meal and tuber peels. The experimental work was carried out in the main laboratory Unit of the Faculty of Agriculture, University of Benin, Ugbowo, Benin City, Edo State. The feed grades of sweet potato (Ipomoea batata L.) leaf meal and tuber peels include 100% Leaf Meal, 100% Tuber peels, 25% Leaf Meal + 75% Tuber peels, 50% Leaf Meal + 50% Tuber peels and 75% Leaf Meal + 25% Tuber peels. Data collected include proximate and mineral composition and were subjected to statistical analysis using Analysis of Variance ANOVA of GenStat Statistical package and means were separated using Duncan Multiple Range Test of the same GenStat package. Based on the findings in this study, it is evident that the proximate composition of sweet potato (Ipomoea batata L.) leaf meal and tuber peels varies significantly across different grades. The highest dry matter content (89.95%) was observed in the 100 tuber peels grade, the 100% Leaf Meal grade showed the highest (14.96%) crude protein content. Moreover, the 100% Leaf Meal grade also indicate the highest (4.737%) ether extract content, which could contribute to energy density in feed formulations while 100%Leaf Meal grade contained the highest (14.450%) crude fiber content, the ash content was higher (13.163) in leaf meal grades compared to tuber peels, indicating differences in mineral composition. Based on the findings of this study, it is recommended to incorporate sweet potato leaf meal and tuber peels in feed formulations, tailored to meet specific dietary requirements. The findings showed that100 Leaf Meal grade consistently gave the highest proximate and
mineral element, 100% Leaf Meal grade is therefore recommended as diet for animal production.
Supervisor(s)
co-supervisor