IDADA AISOSA DANIEL

Vernonia Amygdalina is a plant renowned for processing several bioactive compounds, some of which may hold promising medicinal properties. Delving into its impact on leukemia presents an opportunity to uncover valuable insights regarding its therapeutic applications in leukemia-related conditions. The objective of this study was to determine the effects of Vernonia Amygdalina leaves extract on RAS and WT1 leukemia gene in male albino Wistar rats. A total of twenty (20) male adult albino Wistar rats were selected into four (4)M groups. The groups were the control group,benzene:2-propanol group, 250mg/kg of Vernonia Amygdalina group, andbenzene:2-propanol with 250mg/kg Vernonia A mygdalina group. RNA extraction and semi-quantitative polymerase chain reaction (PCR) were used to isolate RNA from rat bone marrow, and complementary DNA was synthesized and was subsequently amplified using polymerase chain reaction. Gel electrophoresis was used to determine the bands of the genes while peripheral blood film and full blood count were carried out by manual and automated methods respectively. The findings revealed that; for WBC (White Blood Cells),there was no significant difference (p=0.881) in group 3 (8.90±3.60) and group 4 (7.03±1.16) when compared to group 1 (8.27±1.91) and group 2 (7.97±0.70).Also, in N(Neutrophils),there was no significant difference (p=0.149) in group 3 (2.50±0.50) and group 4 (6.00±2.04) when compared to group 1(10.00±0.58) and group 2 (9.00±2.65).L (Lymphocytes)also showed no significant difference (p=0.183) in group 3 (91.00±1.00) and group 4 (88.00±2.71) when compared to group 1(82.00±1.53) and group 2 (85.67±2.60).The M (Monocytes)also showed no significant difference (p=0.523) in group 3 (5.00±0.00) and group 4 (5.00±0.91) when compared to group 1 (6.67±1.76) and group 2 (4.33±0.33). E (Eosinophils)showedno significant difference (p=0.987) in group 3 (1.50±0.50) and group 4 (1.33±0.33) when compared to group 1 (1.33±0.33) and group 2 (1.33±0.33). The RBC (Red Blood Cells)also showed no significant difference(p=0.531) in group 3 (6.32±0.49) and group 4 (6.53±0.21) when compared to group 1 (6.02±0.78) and group 2 (6.99±0.24).Hb (Hemoglobin)also showed no significant difference (p=0.226) in group 3 (12.95±0.25) and group 4 (13.30±0.38) when compared to group 1 (12.50±1.40) and group 2 (15.03±0.64).PLT (Platelets)showed no significant difference (p=0.649) in group 3 (1010.50±252.50) and group 4 (27536.50±26837.19) when compared to group 1 (1356.00±705.72) and 2 (1111.67±193.69), except for a notable outlier in group 4 (27536.50±26837.19). This study concludes that Vernonia Amygdalina did not have any significant effect on leukemia gene RAS and WT1 expression. These results suggest that Vernonia amygdalina could not influence the expression of the leukemia gene RAS and WT1.