RISK ASSESSMENT AND ANTIBIOTICS RESISTANCE OF E.Coli AND Salmonella SPP IN ABATTOIR WASTEWATER IN BENIN CITY.
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Abstract
The research was carried out to evaluate the risk assessment and antibiotic resistant of E.coli and Salmonella spp in abattoir waste water in Benin city. Standard bacteriological methods were used to enumerate the total bacterial count (E.coli and Salmonella spp) of abattoir waste water using pour plate methods after serial dilution. The bacterial isolates were characterized and identified using morphological and biochemical methods and sugar fermentation test. The percentage distribution and frequency of the isolates were evaluated using statistical method. From the result the total Escherichia coli colony count in cfu/ml ranges from 0.7 x 103 – 0.8 x 103 (cfu/ml) while that of Salmonella spp ranges from 0.7 x 103 – 0.8 x 103 (cfu/ml). Following the cultural, morphological and biochemical characteristics of bacteria from abattoir waste water samples, the result revealed that Salmonella enterica, 2 Escherichia coli strains, Salmonella spp, and Salmonella typhi. The antibiotic sensitivity test revealed that all isolates found in this study were multiple antibiotic resistant bacteria. The multiple antibiotic resistant index of these isolates ranges from 0.5 to 0.525 as against the recommended 0.2 by WHO.
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