THE SURVIVAL STUDIES OF Lactobacillus gasseri IN THE PRESENCE OF SOME SUPPOSITORY BASES AND Escherichia coli .
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Abstract
Introduction: The survival and interaction of probiotic microorganisms in pharmaceutical carriers are critical in determining their therapeutic efficacy. This study investigated the survival pattern of Lactobacillus gasseri in the presence of selected suppository bases and Escherichia coli , with the aim of identifying a suitable base that enhances probiotic viability while inhibiting pathogenic growth. Methods: Pure cultures of Lactobacillus gasseri and Escherichia coli were obtained and characterized through Gram staining and biochemical tests. Survival studies were conducted
by inoculating the organisms in media containing different suppository bases— glycerogelatin, Polyethylene Glycol (POLYETHYLENE GLYCOL), and Theobroma base—and monitoring their growth patterns over time. The regression equations for the
growth curves were determined as follows: control Lactobacillus gasseri, y = 6.81 + 0.18x; glycerogelatin, Escherichia coli , y = 4.31 − 0.06x; POLYETHYLENE GLYCOL, Escherichia coli , y = 3.27 + 0.24x; Theobroma base, Escherichia coli , y = 6.93 + 0.23x. Statistical analyses were performed to evaluate significance (p < 0.05)
by inoculating the organisms in media containing different suppository bases— glycerogelatin, Polyethylene Glycol (POLYETHYLENE GLYCOL), and Theobroma base—and monitoring their growth patterns over time. The regression equations for the
growth curves were determined as follows: control Lactobacillus gasseri, y = 6.81 + 0.18x; glycerogelatin, Escherichia coli , y = 4.31 − 0.06x; POLYETHYLENE GLYCOL, Escherichia coli , y = 3.27 + 0.24x; Theobroma base, Escherichia coli , y = 6.93 + 0.23x. Statistical analyses were performed to evaluate significance (p < 0.05)
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