DOTUN ANDREW AWOLEYE

Background: Ear infections, also known as otitis media, are a common type of bacterial infection that mostly affect children. Plasmid profiling is a molecular technique used to identify and characterize the types of plasmids carried by bacterial isolates. This technique has a wide range of applications in microbiology, including the study of bacterial epidemiology, antibiotic resistance, and gene transfer. It can provide valuable information about the genetic diversity of bacterial populations and the mechanisms by which bacteria acquire and transfer resistance genes. Methods: The study evaluated a hundred and twenty seven patients from the Ear, Nose and Throat department at the University of Benin Teaching Hospital, Benin city. Patient’s data collected were age, gender, alcohol history, smoking history, medical history, Frequency of symptoms, ears affected, use of cotton bud, ear cleaning frequency among others. Isolates obtained were subjected to antimicrobial susceptibility testing and plasmid curing with 0.1mL and 0.4mL Ethidium bromide as the curing agent using standard agar disc diffusion method. Results: A total of 73.68% of our study participants were female, and 14.47% were male. Participants aged 21-25 had the highest occurrence (34.6%), linked to the patients ear cleaninghabits. The prevalence of Staphylococcus spp and Pseudomonas aeruginosa from both aerobic and anaerobic Isolate was the highest (49.33% and 47.06%) in the bacterial isolates obtained from study participants after incubation.The most prevalent mult-idrug resistant isolate was Staphylococcus aureus (25%) for aerobic isolate and Pseudomonas spp for anaerobic Isolate. Fluoroqinolones was the most effective antibiotics used for antimicrobial susceptibility testing. Post
curing with 0.1mg/ml and 0.4mg/ml Ethidium bromide,33.33%,100% and 0% was cured after further antimicrobial susceptibility testing with Fluoroqinolones, Macrolides and Penicillin) respectively. Conclusion:The presence of plasmid-mediated multidrug resistance genes in bacteria isolates from patients with ear infection underscores the need for antibiograms and responsible antibiotic usage in the management of ear infection.