CHUKWUEMEKE, KESTER. C

Free radicals have been implicated in the occurrence of oxidative stress. They have also been found to be important in the pathophysiology of a number of disease conditions. This therefore, underlies the need for very effective antioxidants. Ficus exasperata has been used traditionally for the treatment and management of numerous disease conditions. Assessment of antioxidant properties creates opportunities for further research into the pharmacological, toxicological properties and clinical relevance of Ficus exasperata. The plant sample was collected, identified, dried, and extracted. Plant sample was assessed for its phytochemical constituents, Total Phenolic Content (TPC) and Total Flavonoid Content (TFC), using spectrophotometric methods. Using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, and Total Antioxidant Capabilities (TAC), the antioxidant properties of the plant extract was assessed using specified methods. Glycosides, alkaloids, flavonoids, phenols were among the phytochemicals observed to be present. Total Phenolic Content (TPC) was -1.82±47 mg GAE/g of exteract, Total Flavonoid Content (TFC) was found to be 47.69±23.2 mg QE/g of extract. The IC50 of ascorbic acid and extract for DPPH scavenging activity was determined to be 1.31 µg/mL and 1.91 µg/mL, respectively. The IC50 for TAC of the extract was determined to be 1.23 µg/mL, while that of the standard (ascorbic acid) was found to be 8.82𝑥10−9 µg/mL.