In-vitro Antioxidant properties of Moringa oleifera Leaf Extract in Management of Oxidative Stress.

Moringa oleifera is widely valued for its medicinal and nutritional importance,particularly its rich content of bioactive compounds with antioxidant properties. This study aimed to evaluate the in vitro antioxidant activity of Moringa oleifera leaf extract and assess its potential role in the management of oxidative stress. Fresh Moringa leaves were collected, washed, weighed, homogenised, and extracted using standard solvent extraction procedures to obtain the crude leaf extract used for analysis.The antioxidant capacity of the extract was examined using three established assays: the Nitric Oxide (NO) scavenging assay, the Ferric Reducing Antioxidant Power (FRAP) assay, and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Ascorbic acid served as the reference standard. Results showed that the Moringa extract exhibited considerable antioxidant activity across all assays, with NO inhibition ranging from 36.34–57.42%, FRAP reducing power from 46.68–58.58%, and DPPH radical scavenging from 57.44–84.21%. Although these values were lower than those of pure ascorbic acid, the extract still demonstrated strong free radical–neutralizing and electron donating abilities.The significant in-vitro activity observed in this study provides scientific justification for its traditional use as a natural antioxidant and suggests that Moringa oleifera is a promising candidate for future in-vivo studies aimed at exploring its therapeutic potential in oxidative stress–related conditions