INVESTIGATION OF THE EFFECT OF ETHANOL EXTRACT OF VERNONIA AMYGDALINA LEAVES ON CARDIOVASCULAR DISEASE RISK FACTORS IN RATS EXPOSED TO DMH
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Abstract
Vernonia amygdalina (Bitter leaf) is a medicinal plant used in traditional medicine for the treatment of various diseases. It possesses different biological activity including antioxidant andantimicrobial activities, antibacterial and analgesic activity. The aim of this study was to
investigate the effect of Vernonia amygdalina on cardiovascular disease risk factors in rats exposed to DMH. Adult Wister rats (n= 30) were collected and randomly assigned to groups (8 rats per groups): normal control, DMH only, Silymarin control, extract only, post treatment 1, post treatment 2, Pre- treatment 1, and Pre- treatment 2. Group 1, was supplied with food and water regularly but no Vernonia amygdalina extract, silymarin antibiotic or 1,2 dimethylhydrazine was administered. Group 2, 1,2 dimethylhydrazine was administered at 3millilitres per kilograms of body, alongside with food and water to induce the heart damage. Loss of appetite, fatigue and dizziness was noted after few days of administration. Group 3, the silymarin an antibiotic at 100ml\kg by weight was administered, DMH was then administered every day for 12 days. Concentrations of lipids: total cholesterol, HDL cholesterol, TAG, High and low-Density lipoprotein cholesterol and very low-Density lipoprotein cholesterol as well as Atherogenic index, Atherogenic coefficient, cardiac risk ratio and cardiac Nitric Oxide was measured in plasma
investigate the effect of Vernonia amygdalina on cardiovascular disease risk factors in rats exposed to DMH. Adult Wister rats (n= 30) were collected and randomly assigned to groups (8 rats per groups): normal control, DMH only, Silymarin control, extract only, post treatment 1, post treatment 2, Pre- treatment 1, and Pre- treatment 2. Group 1, was supplied with food and water regularly but no Vernonia amygdalina extract, silymarin antibiotic or 1,2 dimethylhydrazine was administered. Group 2, 1,2 dimethylhydrazine was administered at 3millilitres per kilograms of body, alongside with food and water to induce the heart damage. Loss of appetite, fatigue and dizziness was noted after few days of administration. Group 3, the silymarin an antibiotic at 100ml\kg by weight was administered, DMH was then administered every day for 12 days. Concentrations of lipids: total cholesterol, HDL cholesterol, TAG, High and low-Density lipoprotein cholesterol and very low-Density lipoprotein cholesterol as well as Atherogenic index, Atherogenic coefficient, cardiac risk ratio and cardiac Nitric Oxide was measured in plasma
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