TOTAL PHENOL AND FLAVINOID CONTENT OF DRIED SEEDS OF SOYA BEAN (Glycine max) AND COTTON SEEDS (Gossypium spp)
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Abstract
This study was carried out to investigate and compare the total phenolic and flavonoid content of dried seeds of Glycine max (soybean) and Gossypium spp. (cottonseed), recognising the growing interest in natural antioxidants and the limited comparative data available for these two commonly seeds. The aim of the study was to determine their antioxidant-related phytochemical
composition and establish which seed possesses higher extractable phenolic and flavonoid levels. Quantitative data were generated from dried, ground seed samples extracted using methanol, and
analysed using standard colorimetric procedures. Total phenolic content was determined using the Folin–Ciocalteu method while total flavonoid content was assessed with the Aluminium chloride
assay, and absorbance readings were obtained spectrophotometrically. The resulting values were processed to obtain mean concentrations and standard error of mean for each parameter measured.
The findings revealed that soybean contained notably higher total phenolic and flavonoid concentrations compared to cottonseed, indicating a stronger antioxidant potential and greater
suitability for nutraceutical or functional food applications. Cottonseed, although lower in these constituents, still showed measurable levels, suggesting possible industrial utilisation after
processing. In conclusion, the study successfully quantified and compared these bioactive compounds, demonstrating clear compositional differences and establishing a basis for further
biochemical and application-focused research.
composition and establish which seed possesses higher extractable phenolic and flavonoid levels. Quantitative data were generated from dried, ground seed samples extracted using methanol, and
analysed using standard colorimetric procedures. Total phenolic content was determined using the Folin–Ciocalteu method while total flavonoid content was assessed with the Aluminium chloride
assay, and absorbance readings were obtained spectrophotometrically. The resulting values were processed to obtain mean concentrations and standard error of mean for each parameter measured.
The findings revealed that soybean contained notably higher total phenolic and flavonoid concentrations compared to cottonseed, indicating a stronger antioxidant potential and greater
suitability for nutraceutical or functional food applications. Cottonseed, although lower in these constituents, still showed measurable levels, suggesting possible industrial utilisation after
processing. In conclusion, the study successfully quantified and compared these bioactive compounds, demonstrating clear compositional differences and establishing a basis for further
biochemical and application-focused research.
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