HISTOMORPHOLOGICAL EFFECTS OF SILDENAFIL (VIAGRA) AS AN APHRODISIAC ON TESTICULAR AND CARDIAC TISSUES IN ADULT ALBINO RATS
Faculty
Department
Year of Publication
Keyword
upload
Publication Type
Abstract
In the course of improving sexual performance, some men have chosen to use aphrodisiac substances as a source of intervention. The study aimed to determine the impact of aphrodisiac (sildenafil: Viagra) use on the testes and heart tissues of Adult Albino rats. Eighteen (18) male albino rats (weighing 200g-220g) were acquired from the animal house of Edo State University. The animals were randomly divided into 3 equal groups A, B and C (n=6/group). Group A served as control administered with feed and water only. Group B, included 6 rats that were orally given Viagra in a dose of 5 mg/kg body weight dissolved in saline daily for 4 successive weeks. Group C, included 6 rats that were orally given Viagra in a dose of 10 mg/kg body weight for 4
successive weeks and then were kept without treatment for 4 weeks. Body weight of experimental animals was checked at week 0 before Viagra administration and last day of drug administration before sacrifice. All experimental rats and control rats were anesthetized using formalin and sacrificed at the end of experimental period. Hematological analysis was done using auto-hematological analyzer while Serial slices were cut using a microtome and stained with hematoxylin and eosin at a thickness of 5 um. Selected tissue sections were photographed and presented as plates. Body weight analysis of the albino rats after four weeks indicated a slight weight difference between the control group and the Viagra administered albino rats. Sildenafil at doses 10mg/kg BW. Hematological parameters reveal there was high significant (p<0.001) decrease in HCT, Twbcs and Granulocytes in the experimental group (41.0 ± 1.66, 2.3 ± 0.55 and 32.8 ± 3.27) when compared with control group (45.6 ± 1.50, 7.4 ± 1.92 and 40.4 ± 1.82) respectively. While monocytes value was highly significant (p<0.001) increase in the experimental group (17.2 ± 2.38 vs 7.4 ± 1.14) when compared with control group. Histological examination showed a general decrease in response to sildenafil administration. While tissue sections of testes collected from rats administrated with 10mg/kg Sildenafil citrate had mild necrosis of both cardiac fibers, seminiferous tubules and the interstitial tissue, congested blood vessels, hypertrophy of the interstitial Leydig cells and degeneration of the spermatogonial cells. Understanding the molecular downstream events involved in long-term PDE5 inhibitor exposure through basic and clinical research can be useful in supervising the application of aphrodisiac substances so as to improve on corrective measures through a short-term administration of aphrodisiac substances at low doses.
successive weeks and then were kept without treatment for 4 weeks. Body weight of experimental animals was checked at week 0 before Viagra administration and last day of drug administration before sacrifice. All experimental rats and control rats were anesthetized using formalin and sacrificed at the end of experimental period. Hematological analysis was done using auto-hematological analyzer while Serial slices were cut using a microtome and stained with hematoxylin and eosin at a thickness of 5 um. Selected tissue sections were photographed and presented as plates. Body weight analysis of the albino rats after four weeks indicated a slight weight difference between the control group and the Viagra administered albino rats. Sildenafil at doses 10mg/kg BW. Hematological parameters reveal there was high significant (p<0.001) decrease in HCT, Twbcs and Granulocytes in the experimental group (41.0 ± 1.66, 2.3 ± 0.55 and 32.8 ± 3.27) when compared with control group (45.6 ± 1.50, 7.4 ± 1.92 and 40.4 ± 1.82) respectively. While monocytes value was highly significant (p<0.001) increase in the experimental group (17.2 ± 2.38 vs 7.4 ± 1.14) when compared with control group. Histological examination showed a general decrease in response to sildenafil administration. While tissue sections of testes collected from rats administrated with 10mg/kg Sildenafil citrate had mild necrosis of both cardiac fibers, seminiferous tubules and the interstitial tissue, congested blood vessels, hypertrophy of the interstitial Leydig cells and degeneration of the spermatogonial cells. Understanding the molecular downstream events involved in long-term PDE5 inhibitor exposure through basic and clinical research can be useful in supervising the application of aphrodisiac substances so as to improve on corrective measures through a short-term administration of aphrodisiac substances at low doses.
Supervisor(s)
co-supervisor